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Phospho-AMPK alpha-1,2 (Thr183, Thr172) Antibody (710099) in ICC/IF
Immunofluorescence analysis of Phospho-AMPK alpha (Thr183, Thr172) was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Phospho-AMPK alpha-1,2 (Thr183, Thr172) Recombinant Rabbit Superclonal™ Antibody (Product # 710... View More
产品信息
710099
产品规格
种属反应
Human,
Non-human primate
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
克隆号
10HCLC
抗原
Peptide corresponding to amino acids 168-178 of human AMPK alpha
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2532562
产品详细信息
This antibody is predicted to react with mouse, rat, non-human primate and Drosophila based on sequence homology.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
靶标信息
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; 5'-AMP-activated protein kinase, catalytic alpha-2 chain; AAPK2; ACACA kinase; Acetyl-CoA carboxylase kinase; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase alpha-2 subunit variant 2; AMP-activated protein kinase alpha-2 subunit variant 3; AMP-activated protein kinase, catalytic, alpha-1; AMPK alpha 1; AMPK alpha 2; AMPK subunit alpha-1; AMPK subunit alpha-2; AMPK-alpha-1 chain; AMPK-alpha-2 chain; HMGCR kinase; Hydroxymethylglutaryl-CoA reductase kinase; pAMPK; protein kinase, AMP-activated, alpha 1 catalytic subunit; protein kinase, AMP-activated, alpha 2 catalytic subunit; Tau-protein kinase PRKAA1
基因别名: AMPK; AMPK1; AMPK2; AMPKa1; AMPKa2; PRKAA; PRKAA1; PRKAA2
UniProt ID: (Human) Q13131, (Human) P54646
Entrez Gene ID: (Human) 5562, (Human) 5563
chromatin binding
protein kinase activity
AMP-activated protein kinase activity
cAMP-dependent protein kinase activity
protein binding
ATP binding
protein C-terminus binding
kinase binding
histone serine kinase activity
metal ion binding
[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity
tau-protein kinase activity
[acetyl-CoA carboxylase] kinase activity
protein serine/threonine kinase activity
protein serine/threonine/tyrosine kinase activity
non-receptor serine/threonine protein kinase
activation of MAPK activity
response to hypoxia
glucose metabolic process
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
fatty acid biosynthetic process
cholesterol biosynthetic process
cell cycle arrest
signal transduction
positive regulation of cell proliferation
lipid biosynthetic process
response to UV
cold acclimation
response to gamma radiation
positive regulation of autophagy
positive regulation of gene expression
response to activity
Wnt signaling pathway
macroautophagy
fatty acid oxidation
response to caffeine
cellular response to nutrient levels
negative regulation of TOR signaling
regulation of peptidyl-serine phosphorylation
histone-serine phosphorylation
intracellular signal transduction
cellular response to glucose starvation
glucose homeostasis
regulation of circadian rhythm
negative regulation of apoptotic process
positive regulation of cholesterol biosynthetic process
positive regulation of glycolytic process
negative regulation of glucosylceramide biosynthetic process
rhythmic process
positive regulation of skeletal muscle tissue development
negative regulation of lipid catabolic process
protein heterooligomerization
fatty acid homeostasis
regulation of vesicle-mediated transport
cellular response to hydrogen peroxide
cellular response to ethanol
cellular response to prostaglandin E stimulus
cellular response to organonitrogen compound
cellular response to hypoxia
response to camptothecin
regulation of signal transduction by p53 class mediator
regulation of energy homeostasis
negative regulation of glucose import in response to insulin stimulus
carnitine shuttle
response to stress
response to muscle activity
positive regulation of macroautophagy
regulation of macroautophagy
cellular response to drug
regulation of fatty acid biosynthetic process
