Invitrogen

Phospho-AS160 (Thr642) Polyclonal Antibody

Phospho-AS160 (Thr642) Antibody in Western Blot (WB)

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Phospho-AS160 (Thr642) Antibody (44-1071G) in WB

Western Blotting. and Mutant Analysis Extracts of 3T3-L1 cells (1-4) or COS cells over-expressing WT AS160 (5, 6) or mutant AS160 T642A (7), treated with PI-3-Kinase inhibitor LY294002 (5) or insulin (1-4, 6-7), were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with the AS160 (pS642... View More

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产品信息

44-1071G

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化 (IHC)

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human AS160 that contains threonine 642. The sequence is conserved in mouse.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533564

靶标信息

AS160 (TBC1D4) is a GTPase-activiting protein for RAB2A, RAB8A, RAB10, and RAB14. The isoform 2 version of this protein promotes the insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane. This movement increases glucose uptake. Mutations associated with the gene cause Diabetes mellitus, non-insulin-dependent, 5 (NIDDM5).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Inhibition of AMPK activity in response to insulin in adipocytes: involvement of AMPK pS485, PDEs, and cellular energy levels.

American journal of physiology. Endocrinology and metabolism

Kopietz F,Rupar K,Berggreen C,Säll J,Vertommen D,Degerman E,Rider MH,Göransson O

44-1071G was used in Western Blot to improve the understanding of how insulin regulates AMP-activated protein kinase activity, and with that, de novo fatty acid synthesis, in adipose tissue.

Tue Sep 01 00:00:00 UTC 2020

A Cell-Autonomous Signature of Dysregulated Protein Phosphorylation Underlies Muscle Insulin Resistance in Type 2 Diabetes.

Cell metabolism

Batista TM,Jayavelu AK,Wewer Albrechtsen NJ,Iovino S,Lebastchi J,Pan H,Dreyfuss JM,Krook A,Zierath JR,Mann M,Kahn CR

44-1071G was used in Western Blotting to reveal a multidimensional network of signaling defects in T2D iMyos going beyond the canonical insulin-signaling cascade, including proteins involved in regulation of Rho GTPases, mRNA splicing and/or processing, vesicular trafficking, gene transcription, and chromatin remodeling.

Tue Nov 03 00:00:00 UTC 2020

Glucose Sensing by Skeletal Myocytes Couples Nutrient Signaling to Systemic Homeostasis.

Molecular cell

Meng ZX,Gong J,Chen Z,Sun J,Xiao Y,Wang L,Li Y,Liu J,Xu XZS,Lin JD

44-1071G was used in Western Blotting to uncover an unexpected mechanism of glucose sensing in skeletal myocytes that contributes to homeostasis and therapeutic action.

Thu May 04 00:00:00 UTC 2017

Skeletal Muscle TRIB3 Mediates Glucose Toxicity in Diabetes and High- Fat Diet-Induced Insulin Resistance.

Diabetes

Zhang W,Wu M,Kim T,Jariwala RH,Garvey WJ,Luo N,Kang M,Ma E,Tian L,Steverson D,Yang Q,Fu Y,Garvey WT

44-1071G was used in Western Blotting to demonstrate that TRIB3 has a pathophysiological role in diabetes and a physiological role in metabolism.

Mon Aug 01 00:00:00 UTC 2016

PKB-Mediated Thr649 Phosphorylation of AS160/TBC1D4 Regulates the R-Wave Amplitude in the Heart.

PloS one

Quan C,Xie B,Wang HY,Chen S

44-1071G was used in western blot to determine that PKB-mediated phosphorylation of AS160/TBC1D4 is one of the major PAS-binding signals in the heart in response to insulin.

Mon Feb 08 00:00:00 UTC 2016

TBC1D1 reduces palmitate oxidation by inhibiting β-HAD activity in skeletal muscle.

American journal of physiology. Regulatory, integrative and comparative physiology

Maher AC,McFarlan J,Lally J,Snook LA,Bonen A

44-1071G was used in Western Blotting to demonstrate that TBC1D1-mediated reduction of muscle fatty acid oxidation appears to occur via inhibition of Β-HAD activity.

Sat Nov 01 00:00:00 UTC 2014

6-Mercaptopurine augments glucose transport activity in skeletal muscle cells in part via a mechanism dependent upon orphan nuclear receptor NR4A3.

American journal of physiology. Endocrinology and metabolism

Liu Q,Zhu X,Xu L,Fu Y,Garvey WT

Published figure using Phospho-AS160 (Thr642) polyclonal antibody (Product # 44-1071G) in Western Blot

Fri Nov 01 00:00:00 UTC 2013

Thr649Ala-AS160 knock-in mutation does not impair contraction/AICAR-induced glucose transport in mouse muscle.

American journal of physiology. Endocrinology and metabolism

Ducommun S,Wang HY,Sakamoto K,MacKintosh C,Chen S

44-1071G was used in western blot to determine the role of AS60-Thr(649) phosphorylation/4-3-3 binding in mediating glucose uptake.

Tue May 15 00:00:00 UTC 2012

Recovered insulin response by 2 weeks of leptin administration in high-fat fed rats is associated with restored AS160 activation and decreased reactive lipid accumulation.

American journal of physiology. Regulatory, integrative and comparative physiology

Stefanyk LE,Gulli RA,Ritchie IR,Chabowski A,Snook LA,Bonen A,Dyck DJ

Fri Jul 01 00:00:00 UTC 2011

Loss of 50% of excess weight using a very low energy diet improves insulin-stimulated glucose disposal and skeletal muscle insulin signalling in obese insulin-treated type 2 diabetic patients.

Diabetologia

Jazet IM,Schaart G,Gastaldelli A,Ferrannini E,Hesselink MK,Schrauwen P,Romijn JA,Maassen JA,Pijl H,Ouwens DM,Meinders AE

Fri Feb 01 00:00:00 UTC 2008

Distinct signals regulate AS160 phosphorylation in response to insulin, AICAR, and contraction in mouse skeletal muscle.

Diabetes

Kramer HF,Witczak CA,Fujii N,Jessen N,Taylor EB,Arnolds DE,Sakamoto K,Hirshman MF,Goodyear LJ

44-1071G was used in western blot to study the relationships among Akt, AMPK alpha2, AS60 phosphorylation, insulin, and AICAR.

Sat Jul 01 00:00:00 UTC 2006

Insulin-mediated phosphorylation of the proline-rich Akt substrate PRAS40 is impaired in insulin target tissues of high-fat diet-fed rats.

Diabetes

Nascimento EB,Fodor M,van der Zon GC,Jazet IM,Meinders AE,Voshol PJ,Vlasblom R,Baan B,Eckel J,Maassen JA,Diamant M,Ouwens DM

Fri Dec 01 00:00:00 UTC 2006

Insulin-mediated phosphorylation of the proline-rich Akt substrate PRAS40 is impaired in insulin target tissues of high-fat diet-fed rats.

Diabetes

Nascimento EB,Fodor M,van der Zon GC,Jazet IM,Meinders AE,Voshol PJ,Vlasblom R,Baan B,Eckel J,Maassen JA,Diamant M,Ouwens DM

Fri Dec 01 00:00:00 UTC 2006

Insulin-mediated phosphorylation of the proline-rich Akt substrate PRAS40 is impaired in insulin target tissues of high-fat diet-fed rats.

Diabetes

Nascimento EB,Fodor M,van der Zon GC,Jazet IM,Meinders AE,Voshol PJ,Vlasblom R,Baan B,Eckel J,Maassen JA,Diamant M,Ouwens DM

Fri Dec 01 00:00:00 UTC 2006

生物信息学

蛋白别名: Akt substrate of 160 kDa; AS160; DKFZp779C0666; OTTHUMP00000040818; RP11-159J2.1; RP11-159J2.3; TBC (Tre-2, BUB2, CDC16) domain-containing protein; TBC Tre-2; TBC1 domain family member 4; TBC1 domain family, member 4

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基因别名: 5930406J04Rik; A930035N22; AS160; AV295684; KIAA0603; NIDDM5; TBC1D4

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UniProt ID: (Human) O60343, (Mouse) Q8BYJ6

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Entrez Gene ID: (Human) 9882, (Mouse) 210789

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功能

GTPase activator activity Rab GTPase binding protein binding scaffold/adaptor protein GTPase-activating protein

参与通路

intracellular protein transport vesicle-mediated transport regulation of vesicle fusion negative regulation of vesicle fusion cellular response to insulin stimulus membrane organization activation of GTPase activity