Invitrogen

Phospho-Acetyl-CoA Carboxylase (Ser79) Polyclonal Antibody

Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody in Western Blot (WB)

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Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody (PA5-85824) in WB

Western blot analysis of Phospho-Acetyl-CoA Carboxylase (Ser79) in untreated (–) and treated (+) 293T whole cell extract using Phospho-Acetyl-CoA Carboxylase (Ser79) polyclonal antibody (Product # PA5-85824) using 30 µg of sample at a dilution of 1:500. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 5% ... View More

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产品信息

PA5-85824

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Carrier-protein conjugated synthetic peptide corresponding to residues around Rat Acetyl-CoA Carboxylase 1 (phospho Ser79).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.12 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2792960

产品详细信息

Keep as concentrated solution.

Predicted reactivity: Mouse (100%), Rat (100%), Pig (100%), Sheep (100%), Rhesus Monkey (100%), Bovine (100%).

Positive Control: Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2, 293T (starvation for 16 hr and 0.5 uM Oligomycin treatment for 30 min).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。