Invitrogen

Phospho-CDK6 (Thr177) Recombinant Superclonal™ Antibody (16HCLC)

Phospho-CDK6 (Thr177) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-CDK6 (Thr177) Antibody (711588) in ICC/IF

For immunofluorescence analysis, T98G cells were fixed and permeabilized for detection of endogenous CDK6 (pT177) using Anti- CDK6 (pT177) Recombinant Rabbit Superclonal™ Antibody (Product # 711588, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using SlowFade® Gold Antifade Mounta... View More

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产品信息

711588

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

2 µg/mL

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Superclonal

类型

Antibody

克隆号

16HCLC

抗原

Peptide corresponding to Human CDK4 (169-175)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2632988

产品详细信息

This antibody is predicted to react with Monkey, Pig, Horse and Bovine

Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

靶标信息

CDK6 functions as a cell-cycle initiator protein. This protein is coexpressed and copurified with CyclinD1. They are critical regulators of cell cycle progression. The prototype member of this family, p34cdc2, and a related protein cdk2, function late in the cycle while cdk4 and cdk6 are critically involved in G1 to S progression. CDK6 is essential for cell proliferation within the dentate gyrus of the hippocampus and the cuventricular zone of the lateral ventricles. Mutations in the gene results in microcephaly 12, primary, autosomal recessive (MCPH12).

仅用于科研。不用于诊断过程。未经明确授权不得转售。