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Phospho-CHK2 (Thr68) Antibody (PA5-78361) in ICC/IF
Immunofluorescence analysis of Serine/threonine-protein kinase Chk2 was performed using 70% confluent log phase HEK-293 cells treated with Etoposide (2uM for 12hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Phospho-CHK2 (Thr68) Polyclonal Antibody... View More
产品信息
PA5-78361
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein encompassing a sequence within the N-terminus region of human Chk2 (phospho Thr68). The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
0.73 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol, 1% BSA
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2736363
产品详细信息
Positive Control: HeLa, 293T, 293T (100µM etoposide for 2hr)
Predicted Reactivity: Pig (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
CHEK2 (CHK2) is a serine/threonine kinase and a component of the DNA damage checkpoint pathway. A mutation in CHK2 has been linked to cancer. CHK2 is activated by DNA damage and phosphorylates several modulators of cell cycle control including tumor suppressor proteins. In addition, Chk2 can phosphorylate BRCA1, allowing BRCA1 to restore survival after DNA damage. Chk2 is a putative tumor suppressor protein, with mutations in to the Chk2 gene linked to Li-Fraumeni syndrome, a familiar cancer usually associated with inherited mutations in TP53. Also, mutations in CHK2 are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Cds1 homolog; Checkpoint kinase 2; Checkpoint like protein CHK2; checkpoint-like protein CHK2; Chk2 (phospho T68); Chk2 (pThr68); CHK2 checkpoint homolog; CHK2 checkpoint homolog (S. pombe); Chk2 phospho; Chk2 phospho T68; del9; EC 2.7.11.1; HuCds 1; Hucds1; kinase Chk2; OTTHUMP00000199044; OTTHUMP00000199045; OTTHUMP00000199064; OTTHUMP00000199115; OTTHUMP00000199116; phospho Chk2 (T68); phospho Thr68 Chk2; RP11-436C9.1; Serine/threonine protein kinase Chk2; Serine/threonine-protein kinase Chk2
基因别名: CDS1; CHEK2; CHK2; hCds1; HuCds1; LFS2; PP1425; RAD53
UniProt ID: (Human) O96017
Entrez Gene ID: (Human) 11200
DNA damage checkpoint
G2/M transition of mitotic cell cycle
replicative cell aging
positive regulation of protein phosphorylation
double-strand break repair
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
cellular response to DNA damage stimulus
DNA damage induced protein phosphorylation
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
intrinsic apoptotic signaling pathway in response to DNA damage
response to gamma radiation
peptidyl-serine phosphorylation
cellular response to drug
regulation of protein catabolic process
signal transduction in response to DNA damage
cellular protein catabolic process
positive regulation of transcription, DNA-templated
protein autophosphorylation
protein stabilization
cell division
negative regulation of cell cycle arrest
signal transduction involved in intra-S DNA damage checkpoint
mitotic spindle assembly
replicative senescence
regulation of signal transduction by p53 class mediator
response to doxorubicin
cellular response to bisphenol A
negative regulation of DNA damage checkpoint
positive regulation of anoikis
