Invitrogen

Phospho-Coronin 1B (Ser2) Polyclonal Antibody

Phospho-Coronin 1B (Ser2) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-Coronin 1B (Ser2) Antibody (PA5-143670) in ICC/IF

Immunocytochemistry analysis of Phospho-Coronin 1B (Ser2) in rabbit spleen fibroblasts treated with Calyculin A. The cells were labeled with rabbit polyclonal Coronin-1B (C-terminus) and Phospho-Coronin 1B (Ser2) polyclonal antibody (Product # PA5-143670), then detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of their respe... View More

Phospho-Coronin 1B (Ser2) Antibody in Western Blot (WB)

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产品信息

PA5-143670

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫细胞化学 (ICC/IF)

1:200

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Coronin-1B (Ser-2) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding serine 2 in human coronin-1B. This sequence has high homology to the conserved site in rat and mouse coronin-1B and in coronin-1A. However, this sequence has low homology to the conserved region in other coronins.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA, 50% glycerol

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2942898

产品详细信息

The antibody detects a 60 kDa* band corresponding to Coronin-1B on SDS-PAGE immunoblots of human A431 or HeLa cells treated with Calyculin A. This band is not observed in control cells and the band observed in Calyculin A-treated cells can be removed with lambda phosphatase treatment.