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Phospho-CtIP (Ser327) Antibody (PA5-37337) in WB
Western blot analysis of Phospho-CtIP (Ser327) in Lane 1: HEK293T cell lysate treated with UV (24h), Lane 2: Raw264.7 cell lysate treated with UV (24h). Samples were incubated with Phospho-CtIP (Ser327) polyclonal antibody (Product # PA5-37337) at a dilution of 1:500.
产品信息
PA5-37337
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic phosphopeptide derived from human CtIP around the phosphorylation site of Serine 327
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.2, with 50% glycerol
内含物
0.02% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2554014
产品详细信息
This antibody detects endogenous protein at a molecular weight of 110 kDa.
Purity is >95% by SDS-PAGE.
靶标信息
CtIP, a 125 kD protein, was originally found interacting with a transcription repressor, CtBP, through the PLDLS motif (CtBP-Interacting Protein) thus suggested a role in transcription. Studies have shown that CtIP also interacts with BRCA1 protein through the c-terminus BRCT domains also suggested that CtIP is a potential tumor suppressor. This CtIP-BRCA1 interaction can be disrupted by DNA damaging agents including UV or gamma-irradiation. Li et al (Nature 406, 210 - 215 (2000)) have shown that ATM phosphorylates CtIP at serine residues 664 and 745, and mutation of these sites abrogates the dissociation of BRCA1 from CtIP, resulting in persistent repression of BRCA1-dependent induction of GADD45 upon ionizing radiation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CTBP-interacting protein; CtIP; DNA endonuclease RBBP8; RBBP-8; retinoblastoma binding protein 8; Retinoblastoma-binding protein 8; Retinoblastoma-interacting protein and myosin-like; RIM; SAE2; Sporulation in the absence of SPO11 protein 2 homolog
基因别名: 9930104E21Rik; COM1; CTIP; JWDS; RBBP-8; RBBP8; RIM; SAE2; SCKL2
UniProt ID: (Human) Q99708, (Mouse) Q80YR6
Entrez Gene ID: (Human) 5932, (Mouse) 225182
single-stranded DNA endodeoxyribonuclease activity
RNA polymerase II repressing transcription factor binding
RNA polymerase II transcription corepressor activity
damaged DNA binding
protein binding
DNA binding
nuclease activity
endonuclease activity
hydrolase activity
phosphoric ester hydrolase activity
T/G mismatch-specific endonuclease activity
retroviral 3' processing activity
transcription cofactor
gene-specific transcriptional regulator
cell cycle checkpoint
G1/S transition of mitotic cell cycle
negative regulation of transcription from RNA polymerase II promoter
double-strand break repair via homologous recombination
DNA double-strand break processing
DNA synthesis involved in DNA repair
strand displacement
blastocyst hatching
DNA replication
DNA repair
nucleotide-excision repair
regulation of transcription from RNA polymerase II promoter
mitotic nuclear division
DNA double-strand break processing involved in repair via single-strand annealing
G2 DNA damage checkpoint
response to estradiol
cell division
meiotic cell cycle
nucleic acid phosphodiester bond hydrolysis
regulation of signal transduction by p53 class mediator
DNA damage checkpoint
transcription from RNA polymerase II promoter
cellular response to DNA damage stimulus
cell cycle
