Invitrogen

Phospho-DAPK1 (Ser308) Polyclonal Antibody

Phospho-DAPK1 (Ser308) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-DAPK1 (Ser308) Antibody (PA5-105788) in ICC/IF

Immunofluorescent analysis of Phospho-DAPK1 (Ser308) in HeLa cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-DAPK1 (Ser308) polyclonal antibody (Product # PA5-105788) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 59... View More

Phospho-DAPK1 (Ser308) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-DAPK1 (Ser308) Antibody in Western Blot (WB)

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产品信息

PA5-105788

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human DAPK1(Accession P53355), corresponding to amino acid residues around phosphorylated Ser308.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

sequential chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2817187

产品详细信息

Antibody detects endogenous levels of DAPK1 only when phosphorylated at Ser308.

靶标信息

DAPK1 is a large, calmodulin-dependent kinase involved in gamma-interferon induced programmed cell death and is a potential tumor suppressor. DAPK1 is involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and authophagy. It regulates both type I apoptotic and type II autophagic cell death signal, depending on the cellular setting. Type I apoptotic cell death is caspase-dependent, type II authophagic cell death is caspase-independent. DAPK1 phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. It also phosphorylates TPM1, STX1A, PRKD1, BECN1, TSC2, RPS6, MYL9, and DAPK3.

仅用于科研。不用于诊断过程。未经明确授权不得转售。