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Phospho-DNA ligase IV (Thr650) Antibody (PA5-105827) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-DNA ligase IV (Thr650) in mouse kidney tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-DNA ligase IV (Thr650) polyclonal antibody (Product # PA5-105827) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
产品信息
PA5-105827
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human LIG4(Accession P49917), corresponding to amino acid residues around phosphorylated Thr650.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
sequential chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2817226
产品详细信息
Antibody detects endogenous levels of DNA Ligase IV only when phosphorylated at Thr650.
靶标信息
The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DNA joinase; DNA ligase 4; DNA ligase IV; DNA repair enzyme; ligase IV, DNA, ATP-dependent; Polydeoxyribonucleotide synthase [ATP] 4; polynucleotide ligase; sealase
基因别名: 5830471N16Rik; LIG4; LIG4S; tiny
UniProt ID: (Human) P49917, (Mouse) Q8BTF7
Entrez Gene ID: (Human) 3981, (Mouse) 319583
single strand break repair
in utero embryonic development
pro-B cell differentiation
DNA ligation
lagging strand elongation
nucleotide-excision repair, DNA gap filling
double-strand break repair
double-strand break repair via nonhomologous end joining
cell cycle
central nervous system development
cell proliferation
response to X-ray
response to gamma radiation
T cell differentiation in thymus
V(D)J recombination
immunoglobulin V(D)J recombination
T cell receptor V(D)J recombination
somatic stem cell population maintenance
negative regulation of neuron apoptotic process
isotype switching
positive regulation of fibroblast proliferation
positive regulation of neurogenesis
DNA ligation involved in DNA recombination
DNA ligation involved in DNA repair
chromosome organization
cell division
neuron apoptotic process
cellular response to lithium ion
DNA biosynthetic process
establishment of integrated proviral latency
double-strand break repair via classical nonhomologous end joining
positive regulation of chromosome organization
DNA replication
DNA repair
DNA recombination
cellular response to DNA damage stimulus
response to ionizing radiation
