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Phospho-EIF2S1 (Ser52) Antibody (PA5-85618) in WB
Western Blot using Phospho-EIF2S1 (Ser52) Polyclonal Antibody (Product # PA5-85618). Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Phospho-EIF2S1 (Ser52) Polyclonal Antibody (Product # PA5-85618) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed... View More
产品信息
PA5-85618
产品规格
种属反应
Human,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Carrier-protein conjugated synthetic peptide corresponding to residues around human eIF2 alpha (phospho Ser52).
偶联物
形式
Liquid
浓度
0.77 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 1% BSA, 20% glycerol
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2792758
产品详细信息
Keep as concentrated solution.
Positive Control: 293T, 293T (25 µg/ml anisomycin treatment for 16hr), HeLa, HeLa (4 µg/ml Tunicamycin treatment for 16hr), HepG2, HepG2 (3 µM Thapsigargin treatment for 12 hr), PC-12 (300 µM Thapsigargin treatment for 8hr).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
EIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner to form the 43S pre-initiation complex. The polypeptide can be phosphorylated by related protein kinases activated in response to stress. Phosphorylated EIF2A inhibits EIF2B activity and prevents guanine nucleotide exchange.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: eIF-2-alpha; EIF-2A; EIF-2alpha; eIF2 alpha; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
基因别名: EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A; EIF2S1
UniProt ID: (Human) P05198, (Rat) P68101
Entrez Gene ID: (Human) 1965, (Rat) 54318
positive regulation of defense response to virus by host
translational initiation
aging
negative regulation of translational initiation in response to stress
cellular response to amino acid starvation
cellular response to heat
cellular response to UV
response to endoplasmic reticulum stress
PERK-mediated unfolded protein response
protein autophosphorylation
transmembrane transport
mitophagy in response to mitochondrial depolarization
xenophagy
positive regulation of neuron death
response to manganese-induced endoplasmic reticulum stress
positive regulation of type B pancreatic cell apoptotic process
translation
regulation of translational initiation in response to stress
