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Phospho-ERK5 (Thr218, Tyr220) Antibody (44-612G) in WB
Extracts prepared from HEK293 cells transiently transfected with plasmids expressing ERK5 kinase domain (ERK5kin) and constitutively activated MEK5D-D were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with the ERK5 (Thr218, Tyr220) antibody for two hours at room temperature in a 3% BSA-TBST bu... View More
产品信息
44-612G
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human,
Mouse,
Non-human primate
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human ERK5 that contains threonine 218 and tyrosine 220. The sequence is conserved in mouse.
偶联物
形式
Liquid
纯化类型
Antigen affinity chromatography
保存液
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2533694
靶标信息
MAPK7 (ERK5) serine/threonine kinase is a member of the MAP kinase family and is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. MAPK7 is activated by mitogen-activated protein kinase 5 (MAP2K5/MEK5) and is involved in downstream signaling processes of various receptors. In response to extracelluar signals, MAPK7 translocates to the cell nucleus where it regulates gene expression by phosphorylating and activating different transcription factors. Gene deletion of MAPK7 in mice results in defective blood vessel and cardiac development leading to embryonic lethality. MAPK7 has been shown to be critical for endothelial function and maintenance of blood vessel integrity. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported. ERK5 is expressed in many adult tissues, abundantly in heart, placenta, lung, kidney and skeletal muscle, but is not detectable in liver.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Big MAP kinase 1; BMK-1; BMK1 kinase; BNhp-1; BNhp1 kinase; ERK-5; Extracellular signal-regulated kinase 5; extracellular-signal-regulated kinase 5; MAP kinase 7; MAPK 7; Mitogen-activated protein kinase 7
基因别名: BMK1; ERK4; ERK5; MAPK7; PRKM7
UniProt ID: (Human) Q13164
Entrez Gene ID: (Human) 5598
MAPK cascade
cell cycle
signal transduction
peptidyl-serine phosphorylation
cAMP-mediated signaling
cell differentiation
negative regulation of cAMP catabolic process
negative regulation of heterotypic cell-cell adhesion
positive regulation of transcription from RNA polymerase II promoter in response to stress
negative regulation of MAP kinase activity
regulation of angiogenesis
positive regulation of transcription from RNA polymerase II promoter
negative regulation of inflammatory response
positive regulation of protein metabolic process
negative regulation of cyclic-nucleotide phosphodiesterase activity
negative regulation of NFAT protein import into nucleus
negative regulation of response to cytokine stimulus
cellular response to hydrogen peroxide
negative regulation of ERK5 cascade
cellular response to growth factor stimulus
cellular response to laminar fluid shear stress
cellular response to transforming growth factor beta stimulus
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
negative regulation of endothelial cell apoptotic process
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
