Invitrogen

Phospho-GCN2 (Thr899) Polyclonal Antibody

View all (19) GCN2 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-105886

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human EIF2AK4(Accession Q9P2K8), corresponding to amino acid residues around phosphorylated Thr899.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2817285

Product Specific Information

Antibody detects endogenous levels of GCN2 only when phosphorylated at Thr899.

Target Information

EIF2AK4 (GCN2) is a metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor-2 in response to low amino acid availability. It plays a role as an activator of the integrated stress response required for adapatation to amino acid starvation. Mutations in the gene lead to pulmonary vennocclusive disease 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: eIF-2-alpha kinase GCN2; Eukaryotic translation initiation factor 2-alpha kinase 4; GCN2 eIF2alpha kinase; GCN2-like protein; general control nonderepressible 2; mGCN2

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Gene Aliases: 2610011M03; EIF2AK4; GCN2; KIAA1338; MGCN2; PVOD2

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UniProt ID: (Human) Q9P2K8, (Mouse) Q9QZ05

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Entrez Gene ID: (Human) 440275, (Mouse) 27103, (Rat) 114859

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Function(s)
tRNA binding protein serine/threonine kinase activity eukaryotic translation initiation factor 2alpha kinase activity ATP binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups eukaryotic initiation factor eIF2 binding aminoacyl-tRNA synthetase non-receptor serine/threonine protein kinase translational protein
Process(es)
positive regulation of defense response to virus by host adaptive immune response T cell activation involved in immune response positive regulation of adaptive immune response regulation of translational initiation protein phosphorylation cell cycle arrest learning long-term memory regulation of translational initiation by eIF2 alpha phosphorylation viral translation negative regulation of translational initiation in response to stress negative regulation of CREB transcription factor activity cellular response to amino acid starvation cellular response to UV eiF2alpha phosphorylation in response to endoplasmic reticulum stress induction by virus of host autophagy negative regulation by host of viral genome replication negative regulation of neuron differentiation negative regulation of translational initiation protein autophosphorylation defense response to virus regulation of feeding behavior regulation of eIF2 alpha phosphorylation by amino acid starvation cellular response to cold positive regulation of translational initiation in response to starvation positive regulation of long-term synaptic potentiation neuron projection extension cellular response to leucine starvation cellular response to starvation positive regulation of gene expression phosphorylation negative regulation of translation endoplasmic reticulum unfolded protein response response to endoplasmic reticulum stress regulation of translational initiation in response to stress
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