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Phospho-GSK3B (Ser9) Antibody (PA1-4688) in WB
Western blot of GSK3B in rat cortical lysate showing specific immunolabeling of a band at ~46 kDa corresponding to Phospho-GSK3B (Ser9) polyclonal antibody (Product # PA1-4688) in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by pre-adsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
产品信息
PA1-4688
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse,
Rat
已发表种属
Amphibian,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser9 of rat GSK3B conjugated to KLH
偶联物
形式
Liquid
纯化类型
Affinity chromatography
保存液
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
内含物
no preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2115231
产品详细信息
This antibody is predicted to react with human, mouse, chicken, bovine, canine, non-human primate, zebrafish and Xenopus based on 100% sequence homology.
In Western blot, this antibody is specific for the ~46kDa GSK3 beta protein phosphorylated at Ser9. It also weakly labels the ~51 kDa GSK3a band due to the high degree of homology between the 2 subunits. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
靶标信息
GSK3B is a proline-directed serine/threonine protein kinase involved in energy metabolism, neuronal cell development and body pattern formation. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. GSK3B is a critical component of the Wnt/beta-catenin signaling pathway, and its activity results in the inhibition of Wnt signaling by mediating the degradation of beta-catenin. GSK3B is also involved in the Hedgehog, Notch, and insulin signaling pathways.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: EC 2.7.11.26; FA; Factor A; Glycogen synthase kinase-3 beta; GSK 3 beta; GSK 3B; GSK-3 beta; GSK-3 beta GSK3B; GSK-3b; GSK3 beta; pp37; Serine/threonine-protein kinase GSK3B
基因别名: 7330414F15Rik; 8430431H08Rik; C86142; GSK-3; GSK-3beta; GSK3; Gsk3b
UniProt ID: (Mouse) Q9WV60, (Rat) P18266
Entrez Gene ID: (Mouse) 56637, (Rat) 84027
nucleotide binding
RNA polymerase II transcription factor binding
p53 binding
protein kinase activity
protein serine/threonine kinase activity
integrin binding
protein binding
ATP binding
beta-catenin binding
transcription factor binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein kinase binding
ubiquitin protein ligase binding
protein kinase A catalytic subunit binding
ionotropic glutamate receptor binding
tau protein binding
tau-protein kinase activity
NF-kappaB binding
re-entry into mitotic cell cycle
epithelial to mesenchymal transition
positive regulation of cell-matrix adhesion
carbohydrate metabolic process
glycogen metabolic process
regulation of gene expression by genetic imprinting
protein phosphorylation
protein export from nucleus
ER overload response
cytoskeleton organization
establishment or maintenance of cell polarity
multicellular organism development
nervous system development
axonogenesis
myoblast fusion
circadian rhythm
cell proliferation
organ morphogenesis
negative regulation of signal transduction
negative regulation of cardiac muscle hypertrophy
positive regulation of peptidyl-threonine phosphorylation
regulation of neuron projection development
negative regulation of neuron projection development
negative regulation of neuron maturation
myotube differentiation
Wnt signaling pathway
phosphorylation
cell migration
peptidyl-serine phosphorylation
hippocampus development
establishment of cell polarity
cell differentiation
negative regulation of protein complex assembly
positive regulation of protein complex assembly
negative regulation of protein binding
positive regulation of protein binding
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
regulation of microtubule-based process
positive regulation of peptidyl-serine phosphorylation
protein localization to microtubule
intracellular signal transduction
cellular response to interleukin-3
positive regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of MAP kinase activity
positive regulation of GTPase activity
hypermethylation of CpG island
canonical Wnt signaling pathway involved in positive regulation of apoptotic process
fat cell differentiation
positive regulation of axon extension
positive regulation of transcription from RNA polymerase II promoter
protein autophosphorylation
positive regulation of protein export from nucleus
regulation of neuronal synaptic plasticity
rhythmic process
negative regulation of dendrite morphogenesis
negative regulation of NFAT protein import into nucleus
canonical Wnt signaling pathway
superior temporal gyrus development
cellular response to mechanical stimulus
extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway
positive regulation of stem cell differentiation
response to lithium ion
response to drug
