Invitrogen

Phospho-GluR1 (Ser845) Polyclonal Antibody

Phospho-GluR1 (Ser845) Antibody in Western Blot (WB)

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Phospho-GluR1 (Ser845) Antibody (OPA1-04118) in WB

Western blot of GluR1 in rat hippocampal lysate showing specific immunolabeling of a ~100 kDa band corresponding to Phospho-GluR1 (Ser845) polyclonal antibody (Product # OPA1-04118) in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (1,200 units for 30 min).

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产品信息

OPA1-04118

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化 (IHC)

1:1,000

免疫组化（冰冻） (IHC (F))

1:1,000

其他PubMed (MISC)

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产品规格

种属反应

Mouse, Rat

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser845 of rat GRIA1 conjugated to KLH

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_325368

产品详细信息

This antibody is predicted to react with human, mouse and non-human primate based on 100% sequence homology.

In Western blot, this antibody detects a ~100 kDa protein representing phospho-GluR1 (pSer845) in rat brain lysate. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.

靶标信息

GluR1 (glutamate receptor 1) is an ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter, L-glutamate, induces a conformation change that leads to the opening of the cation channel and converts the chemical signal to an electrical impulse. Then, the receptor desensitizes rapidly and enters a transiet inactive state characterized by the presence of a bound agonist. In the presence of CACNG4, CACNG7, or CACNG8 GluR1 shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

GSK3α, not GSK3β, drives hippocampal NMDAR-dependent LTD via tau-mediated spine anchoring.

The EMBO journal

Draffin JE,Sánchez-Castillo C,Fernández-Rodrigo A,Sánchez-Sáez X,Ávila J,Wagner FF,Esteban JA

OPA1-04118 was used in Western Blotting to investigate the importance of isoform-specific functions of GSK3 in synaptic plasticity.

Fri Jan 15 00:00:00 UTC 2021

KIF13A drives AMPA receptor synaptic delivery for long-term potentiation via endosomal remodeling.

The Journal of cell biology

Gutiérrez Y,López-García S,Lario A,Gutiérrez-Eisman S,Delevoye C,Esteban JA

Published figure using Phospho-GluR1 (Ser845) polyclonal antibody (Product # OPA1-04118) in Western Blot

Mon Jun 07 00:00:00 UTC 2021

Sex-Specific Anxiety and Prefrontal Cortex Glutamatergic Dysregulation Are Long-Term Consequences of Pre-and Postnatal Exposure to Hypercaloric Diet in a Rat Model.

Nutrients

Rivera P,Tovar R,Ramírez-López MT,Navarro JA,Vargas A,Suárez J,Fonseca FR

OPA1-04118 was used in Western Blotting to conclude that a hypercaloric maternal diet induces sex-dependent anxiety, associated with alterations in both glutamatergic and cannabinoid signaling in the prefrontal cortex, which are accentuated with the continuation of the palatable diet during the life of the offspring.

Fri Jun 19 00:00:00 UTC 2020

Sex-Specific Anxiety and Prefrontal Cortex Glutamatergic Dysregulation Are Long-Term Consequences of Pre-and Postnatal Exposure to Hypercaloric Diet in a Rat Model.

Nutrients

Rivera P,Tovar R,Ramírez-López MT,Navarro JA,Vargas A,Suárez J,Fonseca FR

Fri Jun 19 00:00:00 UTC 2020

Cocaine-induced behavioral sensitization decreases the expression of endocannabinoid signaling-related proteins in the mouse hippocampus.

European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology

Blanco E,Galeano P,Palomino A,Pavón FJ,Rivera P,Serrano A,Alen F,Rubio L,Vargas A,Castilla-Ortega E,Decara J,Bilbao A,de Fonseca FR,Suárez J

OPA1-04118 was used in western blot to determine if the hippocampal gene/protein expression of relevant glutamate signaling components, cannabinoid type 1 receptor, and endocannabinoid metabolic enzymes were altered following acute and/or repeated cocaine administration

Tue Mar 01 00:00:00 UTC 2016

Attenuation of cocaine-induced conditioned locomotion is associated with altered expression of hippocampal glutamate receptors in mice lacking LPA1 receptors.

Psychopharmacology

Blanco E,Bilbao A,Luque-Rojas MJ,Palomino A,Bermúdez-Silva FJ,Suárez J,Santín LJ,Estivill-Torrús G,Gutiérrez A,Campos-Sandoval JA,Alonso-Carrión FJ,Márquez J,de Fonseca FR

OPA1-04118 was used in western blot to investigate the contribution of LPA1 receptors to drug abuse

Thu Mar 01 00:00:00 UTC 2012

生物信息学

蛋白别名: AI853806; AMPA-selective glutamate receptor 1; GluA1; GLUH 1; GluR K1; GluR-1; GluR-A; GluR-K1; GluR1; GluRK1; glutamate receptor; Glutamate receptor 1; glutamate receptor A; Glutamate receptor ionotropic, AMPA 1; glutamate receptor subunit GluR1; glutamate receptor, ionotropic, AMPA1 (alpha 1); GPRC1A; Gria 1; GRM1A; HGBR1; HIPA; MGC13325; MGC133252; NMDA1; NMDAR1; NR1; OTTHUMP00000160643; OTTHUMP00000165781; OTTHUMP00000224241; OTTHUMP00000224242; OTTHUMP00000224243; RP11-350O14.1

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基因别名: 2900051M01Rik; Glr-1; Glr1; GluA1; Glur-1; GluR-A; gluR-K1; Glur1; GluRA; Gria1; HIPA1

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UniProt ID: (Rat) P19490, (Mouse) P23818

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Entrez Gene ID: (Rat) 50592, (Mouse) 14799

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