Invitrogen

Phospho-Histone H1.5 (Ser17) Polyclonal Antibody

Phospho-Histone H1.5 (Ser17) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-Histone H1.5 (Ser17) Antibody (PA5-112445) in ICC/IF

Immunocytochemichal analysis of Phospho Histone H1.5 (Ser17) in HepG2 cells using a Polyclonal antibody (Product # PA5-112445) at a dilution of 1:100. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H... View More

Phospho-Histone H1.5 (Ser17) Antibody in Western Blot (WB)

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产品信息

PA5-112445

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:20-1:200

酶联免疫吸附实验 (ELISA)

Assay-dependent

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Peptide sequence around site of Phospho-Ser (17) derived from Human Histone H1.5

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.05 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.03% ProClin 300

保存条件

-20°C or -80°C if preferred

运输条件

Wet ice

RRID

AB_2867180

靶标信息

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family.

仅用于科研。不用于诊断过程。未经明确授权不得转售。