Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Phospho-IKK alpha/beta (Ser176, Ser180) Recombinant Rabbit Monoclonal Antibody (7H17L17)
图: 1 / 5
Phospho-IKK alpha/beta (Ser176, Ser180) Antibody (701643) in ICC/IF
Time course showing induction of TNF-a signaling cascade upon treatment: Cellular localization of proteins in the NF-KB signaling pathway was detected upon treatment of HeLa cells with TNF-a (50 ng/mL) for 5, 10 and 25 min, respectively. Fixed and permeabilized cells were stained with Anti-IKK alpha/beta (pSpS176/180) Recombinant Rabbit Monoclonal Antibody (Product # 701643, 1 µg/mL) or Anti-NF-kB Mouse Mon... View More
产品信息
701643
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
7H17L17
抗原
Phosphopeptide corresponding to human IKK alpha/beta Ser176/180 (aa 173-182)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2532498
产品详细信息
This antibody is predicted to react with Dog, Rabbit, Pig, Mouse, Rat, Bovine and Goat.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
IKK alpha/beta is encoded by CHUK (conserved helix-loop-helix ubiquitous kinase) and IKKB (inhibitor of kappa light polypeptide gene enhancer in B-cells) gene located in chromosome 10 and 8 respectively in humans. Upon stimulation by agents that activate the NF-kB pathway such as TNF alpha, the two subunits of IKK complex, IKK alpha and IKK -beta alongside a regulator NEMO (IKK gamma) phosphorylates IkB proteins. This targets the later for ubiquitin mediated degradation and phosphorylation of NF-kB leading to subsequent translocation to the nucleus. Phosphorylation at serine 176 and 180 located in the activation loop causes a confirmation change that is required for the activation of the kinases.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: Conserved helix-loop-helix ubiquitous kinase; EC 2.7.11.10; I-kappa-B kinase 1; I-kappa-B kinase 2; I-kappa-B kinase alpha; I-kappa-B kinase-alpha; I-kappa-B-kinase beta; IkappaB kinase; IkB kinase alpha subunit; IKK-1; IKK-2; IKK-a kinase; IKK-B; Inhibitor of nuclear factor kappa-B kinase subunit alpha; Inhibitor of nuclear factor kappa-B kinase subunit beta; NFKBIKA; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase alpha; Nuclear factor NF-kappa-B inhibitor kinase beta; Nuclear factor NFkappaB inhibitor kinase alpha; Serine/threonine protein kinase IKBKB; TCF-16; TCF17; Transcription factor 16
基因别名: CHUK; IKBKA; IKBKB; IKK-alpha; IKK-beta; IKK1; IKK2; IKKA; IKKB; IMD15; NFKBIKA; NFKBIKB; TCF16
UniProt ID: (Human) O15111, (Human) O14920
Entrez Gene ID: (Human) 1147, (Human) 3551
protein kinase activity
protein binding
ATP binding
IkappaB kinase activity
protein homodimerization activity
protein heterodimerization activity
scaffold protein binding
protein serine/threonine kinase activity
protein kinase binding
non-receptor serine/threonine protein kinase
protein modifying enzyme
stimulatory C-type lectin receptor signaling pathway
skeletal muscle contraction
protein phosphorylation
inflammatory response
immune response
I-kappaB kinase/NF-kappaB signaling
I-kappaB phosphorylation
Rho protein signal transduction
response to virus
response to toxic substance
anatomical structure morphogenesis
response to acetate
regulation of tumor necrosis factor-mediated signaling pathway
response to lipopolysaccharide
response to hydroperoxide
TRIF-dependent toll-like receptor signaling pathway
NIK/NF-kappaB signaling
Fc-epsilon receptor signaling pathway
response to drug
positive regulation of I-kappaB kinase/NF-kappaB signaling
response to amino acid
innate immune response
positive regulation of transcription from RNA polymerase II promoter
T cell receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
striated muscle cell differentiation
stress-activated MAPK cascade
nucleotide-binding oligomerization domain containing signaling pathway
cellular response to tumor necrosis factor
positive regulation of cell proliferation
positive regulation of neuron projection development
cortical actin cytoskeleton organization
neuron projection development
tumor necrosis factor-mediated signaling pathway
negative regulation of myosin-light-chain-phosphatase activity
regulation of phosphorylation
serine phosphorylation of STAT protein
negative regulation of apoptotic process
positive regulation of transcription, DNA-templated
interleukin-1-mediated signaling pathway
response to fatty acid
establishment of protein localization to plasma membrane
positive regulation of neuron death
regulation of establishment of endothelial barrier
negative regulation of bicellular tight junction assembly
