Invitrogen

Phospho-IR/IGF1R beta (Tyr1135, Tyr1136, Tyr1150, Tyr1151) Recombinant Rabbit Monoclonal Antibody (S.326.5)

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-15148

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

proprietary

Class

Recombinant Monoclonal

Type

Antibody

Clone

S.326.5

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding pTyr1135/1136 of human IGF-I receptor ß

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

243 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10982593

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other related tyrosine-phosphorylated tyrosine kinases.

Target Information

Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues. Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites. Phosphorylation of these three tyrosine residues is necessary for kinase activation. Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation. Autophosphorylation begins with phosphorylation at Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD220; CD221; IGF-I receptor; Insulin receptor; insulin receptor preproprotein; Insulin receptor subunit alpha; Insulin receptor subunit beta; Insulin-like growth factor 1 receptor; Insulin-like growth factor 1 receptor alpha chain; Insulin-like growth factor 1 receptor beta chain; Insulin-like growth factor I receptor; IR; line 186; soluble IGF1R variant 1; soluble IGF1R variant 2

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Gene Aliases: 4932439J01Rik; A330103N21Rik; CD220; CD221; D630014A15Rik; D930020L01; HHF5; hyft; IGF-1R; IGF1R; IGFIR; IGFIRC; IGFR; Igfr1; INSR; IR; IR-A; IR-B; JTK13

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UniProt ID: (Human) P08069, (Human) P06213, (Rat) P24062, (Mouse) Q60751, (Mouse) P15208

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Entrez Gene ID: (Human) 3480, (Human) 3643, (Rat) 25718, (Mouse) 16001, (Rat) 24954, (Mouse) 16337

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Function(s)
protein tyrosine kinase activity insulin-like growth factor-activated receptor activity insulin receptor binding protein binding insulin-like growth factor binding ATP binding insulin-like growth factor I binding identical protein binding phosphatidylinositol 3-kinase binding insulin binding insulin receptor substrate binding receptor signaling protein tyrosine kinase activity insulin-activated receptor activity insulin-like growth factor receptor binding GTP binding protein phosphatase binding lipoic acid binding insulin-like growth factor II binding protein complex binding 3-phosphoinositide-dependent protein kinase binding PTB domain binding G-protein alpha-subunit binding nucleotide binding protein kinase activity transmembrane receptor protein tyrosine kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding protein domain specific binding
Process(es)
activation of MAPK activity negative regulation of protein phosphorylation positive regulation of protein phosphorylation heart morphogenesis carbohydrate metabolic process regulation of transcription, DNA-templated G-protein coupled receptor signaling pathway positive regulation of cell proliferation insulin receptor signaling pathway epidermis development male gonad development response to manganese ion regulation of hydrogen peroxide metabolic process positive regulation of glycoprotein biosynthetic process negative regulation of gene expression response to activity peptidyl-tyrosine phosphorylation transformation of host cell by virus signal transduction by protein phosphorylation male sex determination adrenal gland development positive regulation of cell migration exocrine pancreas development activation of protein kinase activity activation of protein kinase B activity response to estradiol negative regulation of transporter activity cellular response to insulin stimulus response to vitamin D response to testosterone response to tumor necrosis factor peptidyl-tyrosine autophosphorylation glucose homeostasis positive regulation of MAPK cascade positive regulation of nitric oxide biosynthetic process fat cell differentiation response to ethanol positive regulation of glycogen biosynthetic process positive regulation of DNA replication positive regulation of glycolytic process positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated regulation of embryonic development positive regulation of glucose import protein autophosphorylation positive regulation of developmental growth protein heterotetramerization response to glucocorticoid positive regulation of meiotic cell cycle positive regulation of protein kinase B signaling positive regulation of respiratory burst cellular response to growth factor stimulus regulation of female gonad development immune response signal transduction phosphatidylinositol 3-kinase signaling negative regulation of apoptotic process regulation of JNK cascade insulin-like growth factor receptor signaling pathway phosphatidylinositol-mediated signaling protein tetramerization inactivation of MAPKK activity protein phosphorylation axonogenesis brain development organ morphogenesis negative regulation of muscle cell apoptotic process establishment of cell polarity mammary gland development neuron projection development positive regulation of cytokinesis response to vitamin E negative regulation of MAPK cascade negative regulation of sequence-specific DNA binding transcription factor activity positive regulation of DNA metabolic process protein heterooligomerization negative regulation of protein kinase B signaling prostate gland epithelium morphogenesis positive regulation of steroid hormone biosynthetic process transmembrane receptor protein tyrosine kinase signaling pathway response to hormone response to glucose response to nutrient levels response to insulin positive regulation of phosphorylation phosphorylation intracellular signal transduction
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