Invitrogen

Phospho-IRS1 (Ser307) Polyclonal Antibody

Phospho-IRS1 (Ser307) Antibody in Western Blot (WB)

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Phospho-IRS1 (Ser307) Antibody (44-813G) in WB

Extracts prepared from untreated 3T3L-1 (1), insulin-stimulated 3T3-L1 (2), or insulin-stimulated HEK293 cells transfected with wild-type human IRS-1 (lane 3-7) were resolved by SDS-PAGE on an 8% polyacrylamide gel and transferred to PVDF.

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产品信息

44-813G

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看7篇已发表文章 7篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Hamster, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human IRS-1 that contains serine 307 (serine 302 in mouse sequence).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Affinity chromatography

保存液

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533766

靶标信息

IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Metformin protects against insulin resistance induced by high uric acid in cardiomyocytes via AMPK signalling pathways in vitro and in vivo.

Journal of cellular and molecular medicine

Jiao Z,Chen Y,Xie Y,Li Y,Li Z

44-813G was used in Western Blot to onitor the impact of metformin on HUA-induced IR and in myocardial tissue of an acute hyperuricaemia mouse model established by potassium oxonate treatment.

Thu Jul 01 00:00:00 UTC 2021

Metformin protects against insulin resistance induced by high uric acid in cardiomyocytes via AMPK signalling pathways in vitro and in vivo.

Journal of cellular and molecular medicine

Jiao Z,Chen Y,Xie Y,Li Y,Li Z

44-813G was used in Western Blot to onitor the impact of metformin on HUA-induced IR and in myocardial tissue of an acute hyperuricaemia mouse model established by potassium oxonate treatment.

Thu Jul 01 00:00:00 UTC 2021

The role of Smad4 in the regulation of insulin resistance, inflammation and cell proliferation in HTR8-Svneo cells.

Cell biochemistry and function

Li L,Bai Y,Du R,Tang L,Li L

44-813G was used in Western Blotting to report that deficiency of Smad4 significantly improves insulin sensitivity, relieving inflamation in IR cellular model; and has a positive effect on cell viability.

Fri Jan 01 00:00:00 UTC 2021

Palmitate Is Increased in the Cerebrospinal Fluid of Humans with Obesity and Induces Memory Impairment in Mice via Pro-inflammatory TNF-α.

Cell reports

Melo HM,Seixas da Silva GDS,Sant'Ana MR,Teixeira CVL,Clarke JR,Miya Coreixas VS,de Melo BC,Fortuna JTS,Forny-Germano L,Ledo JH,Oliveira MS,Figueiredo CP,Pardossi-Piquard R,Checler F,Delgado-García JM,Gruart A,Velloso LA,Balthazar MLF,Cintra DE,Ferreira ST,De Felice FG

44-813G was used in Western Blotting to investigate the effects of palmitate, a saturated fatty acid present at high amounts in fat-rich diets, in the brain.

Tue Feb 18 00:00:00 UTC 2020

Chenodeoxycholic Acid Ameliorates AlCl₃-Induced Alzheimer's Disease Neurotoxicity and Cognitive Deterioration via Enhanced Insulin Signaling in Rats.

Molecules (Basel, Switzerland)

Bazzari FH,Abdallah DM,El-Abhar HS

44-813G was used in Western Blotting to show CDCA was able to improve insulin sensitivity in the hippocampi of AlCl3-treated rats, which highlights its potential in AD management.