Invitrogen

Phospho-IRS1 (Ser307) Polyclonal Antibody

Phospho-IRS1 (Ser307) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-IRS1 (Ser307) Antibody (PA1-1054) in ICC/IF

Immunofluorescence analysis of Phospho-IRS1 pSer307 was done on 70% confluent log phase T-47D cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Phospho-IRS1 pSer307 Rabbit Polyclonal Antibody (Product # PA1-1054) at 1 µg/mL in 1% BSA and incubated for ... View More

Phospho-IRS1 (Ser307) Antibody in Western Blot (WB)

Phospho-IRS1 (Ser307) Antibody in Flow Cytometry (Flow)

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产品信息

PA1-1054

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

查看4篇已发表文章 4篇已发表文章

免疫细胞化学 (ICC/IF)

1-2 µg/mL

流式细胞分析 (Flow)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic phosphopeptide corresponding to residues E(301) S I T A T (pS) P A S M V G G K(315) of human IRS-1.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_325022

产品详细信息

PA1-1054 detects phosphorylated IRS-1 (Ser307) in mouse and human cells.

PA1-1054 has been successfully used in Western Blot procedures. By WB, this antibody detects an ~170 kDa protein representing phospho-IRS-1 (Ser307) from 3T3 L1 cells treated with insulin for 10 minutes.

The PA1-1054 immunogen is a synthetic phosphopeptide corresponding to residues E(301) S I T A T (pS) P A S M V G G K(315) of human IRS-1. This peptide is 100% conserved in rats and mice. This peptide (Cat. #PEP-184) is available for use in neutralization and control experiments.

靶标信息

IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Hepatocyte Smoothened Activity Controls Susceptibility to Insulin Resistance and Nonalcoholic Fatty Liver Disease.

Cellular and molecular gastroenterology and hepatology

Chen T,Dalton G,Oh SH,Maeso-Diaz R,Du K,Meyers RA,Guy C,Abdelmalek MF,Henao R,Guarnieri P,Pullen SS,Gregory S,Locker J,Brown JM,Diehl AM

PA1-1054 was used in Western Blot to identify a plausible mechanism whereby inactivation of Smo stimulated the mTORC1-SREBP1c signaling axis, which promoted lipogenesis while inhibiting the hepatic insulin cascade.

Tue Mar 21 00:00:00 UTC 2023

The E3 ubiquitin-protein ligase Trim31 alleviates non-alcoholic fatty liver disease by targeting Rhbdf2 in mouse hepatocytes.

Nature communications

Xu M,Tan J,Dong W,Zou B,Teng X,Zhu L,Ge C,Dai X,Kuang Q,Zhong S,Lai L,Yi C,Tang T,Zhao J,Wang L,Liu J,Wei H,Sun Y,Yang Q,Li Q,Lou D,Hu L,Liu X,Kuang G,Luo J,Xiong M,Feng J,Zhang C,Wang B

PA1-1054 was used in Western Blot to suggest that Trim31 is an endogenous inhibitor of Rhbdf2 and downstream cascades in the pathogenic process of steatohepatitis and that it may serve as a feasible therapeutical target for the treatment of NAFLD/NASH and associated metabolic disorders.

Fri Feb 25 00:00:00 UTC 2022

The E3 ubiquitin-protein ligase Trim31 alleviates non-alcoholic fatty liver disease by targeting Rhbdf2 in mouse hepatocytes.

Nature communications

Xu M,Tan J,Dong W,Zou B,Teng X,Zhu L,Ge C,Dai X,Kuang Q,Zhong S,Lai L,Yi C,Tang T,Zhao J,Wang L,Liu J,Wei H,Sun Y,Yang Q,Li Q,Lou D,Hu L,Liu X,Kuang G,Luo J,Xiong M,Feng J,Zhang C,Wang B

Fri Feb 25 00:00:00 UTC 2022

Mesenchymal stem cell conditioned medium ameliorates diabetic serum-induced insulin resistance in 3T3-L1 cells.

Chronic diseases and translational medicine

Sanap A,Bhonde R,Joshi K

PA1-1054 was used in Flow Cytometry to examine the ability of diabetic serum (DS) to induce IR and investigate whether adipose-derived mesenchymal stem cell conditioned medium (ADMSC-CM) reverses DS-induced IR.

Mon Mar 01 00:00:00 UTC 2021

Mesenchymal stem cell conditioned medium ameliorates diabetic serum-induced insulin resistance in 3T3-L1 cells.

Chronic diseases and translational medicine

Sanap A,Bhonde R,Joshi K

Mon Mar 01 00:00:00 UTC 2021

Enhanced gastrointestinal expression of cytosolic malic enzyme (ME1) induces intestinal and liver lipogenic gene expression and intestinal cell proliferation in mice.

PloS one

Al-Dwairi A,Brown AR,Pabona JM,Van TH,Hamdan H,Mercado CP,Quick CM,Wight PA,Simmen RC,Simmen FA

PA1-1054 was used in western blot to identify the role of intestinal cytosolic malic enzyme 1

Tue Jan 19 00:00:00 UTC 2016

The hyperglycemia-induced inflammatory response in adipocytes: the role of reactive oxygen species.

The Journal of biological chemistry

Lin Y,Berg AH,Iyengar P,Lam TK,Giacca A,Combs TP,Rajala MW,Du X,Rollman B,Li W,Hawkins M,Barzilai N,Rhodes CJ,Fantus IG,Brownlee M,Scherer PE

PA1-1054 was used in western blot to study the important role of reactive oxygen species in the hyperglycemia-induced inflammatory response in adipocytes.