Invitrogen

Phospho-IRS1 (Ser616) Polyclonal Antibody

5 Published Figures

13 References

View all (73) IRS1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Published Figures (5)

Phospho-IRS1 (Ser616) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 8

Phospho-IRS1 (Ser616) Antibody (44-550G) in IHC (P)

Immunohistochemistry analysis of Phospho-IRS1 (pS616) showing staining in the nucleus of paraffin-embedded human breast carcinoma tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min ... View More

Phospho-IRS1 (Ser616) Antibody in Western Blot (WB)

Phospho-IRS1 (Ser616) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Phospho-IRS1 (Ser616) Antibody in Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin) (ICC/IF, IHC, IHC (P))

Phospho-IRS1 (Ser616) Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

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Product Details

44-550G

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of IRS-1 that contains serine 616. The sequence is conserved in many species including human, mouse, and rat

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533678

Product Specific Information

Purified from rabbit serum by sequential epitope-specific chromatography, this product contains enough material for 10 mini-blots. This antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated insulin receptor substrate-1 (IRS-1). The final product is generated by affinity chromatography using IRS-1 derived peptide that is phosphorylated at serine 616.

The antibody has been used in western blotting. Previous lots of this antibody have been used in immunohistochemistry. In western analysis the positive control used was human embryonic kidney cell line transfected with a vector encoding IRS-1 (293T) and stimulated with a phorbol ester, TPA.

Target Information

IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Brain Insulin Signaling is Associated with Late-Life Cognitive Decline.

Aging and disease

Tong H,Capuano AW,Carmichael OT,Gwizdala KL,Bennett DA,Ahima RS,Arnold SE,Arvanitakis Z

44-550G was used in Immunohistochemistry to provide novel evidence for an association between brain insulin signaling and late-life cognitive decline.

Tue Oct 01 00:00:00 UTC 2024

Induction of Brain Insulin Resistance and Alzheimer's Molecular Changes by Western Diet.

International journal of molecular sciences

Mietelska-Porowska A,Domańska J,Want A,Więckowska-Gacek A,Chutorański D,Koperski M,Wojda U

44-550G was used in Western Blotting to suggest that WD promotes the development of AD and may be considered not only a risk factor, but also a modifiable trigger of AD.

Mon Apr 25 00:00:00 UTC 2022

Deletion of serine racemase reverses neuronal insulin signaling inhibition by amyloid-β oligomers.

Journal of neurochemistry

Zhou J,Zhang Z,Yang Y,Liao F,Zhou P,Wang Y,Zhang H,Jiang H,Alinejad T,Shan G,Wu S

44-550G was used in Western Blot to enhance insulin signaling which is inhibited at the early stage of the AD brain.

Sat Oct 01 00:00:00 UTC 2022

Altered Insulin Receptor Substrate 1 Phosphorylation in Blood Neuron-Derived Extracellular Vesicles From Patients With Parkinson's Disease.

Frontiers in cell and developmental biology

Chou SY,Chan L,Chung CC,Chiu JY,Hsieh YC,Hong CT

44-550G was used in Immunoprecipitation to suggest the association between dysinsulin signaling pathway with PD.

Sat Nov 04 00:00:00 UTC 2023

Central insulin dysregulation and energy dyshomeostasis in two mouse models of Alzheimer's disease.

Neurobiology of aging

Velazquez R,Tran A,Ishimwe E,Denner L,Dave N,Oddo S,Dineley KT

44-550G was used in Western Blotting to probe for peripheral insulin resistance, central nervous system insulin sensitivity and energy homeostasis in Tg2576 and 3xTg-Alzheimer's disease mice.

Sun Oct 01 00:00:00 UTC 2017

Insulin and IGF1 signalling pathways in human astrocytes in vitro and in vivo; characterisation, subcellular localisation and modulation of the receptors.

Molecular brain

Garwood CJ,Ratcliffe LE,Morgan SV,Simpson JE,Owens H,Vazquez-Villaseñor I,Heath PR,Romero IA,Ince PG,Wharton SB

44-550G was used in western blot to study the insulin/IGF1 signaling pathway in human astrocytes.

Sat Aug 22 00:00:00 UTC 2015

Insulin and IGF1 signalling pathways in human astrocytes in vitro and in vivo; characterisation, subcellular localisation and modulation of the receptors.

Molecular brain

Garwood CJ,Ratcliffe LE,Morgan SV,Simpson JE,Owens H,Vazquez-Villaseñor I,Heath PR,Romero IA,Ince PG,Wharton SB

44-550G was used in western blot to study the insulin/IGF1 signaling pathway in human astrocytes.

Sat Aug 22 00:00:00 UTC 2015

Insulin and IGF1 signalling pathways in human astrocytes in vitro and in vivo; characterisation, subcellular localisation and modulation of the receptors.

Molecular brain

Garwood CJ,Ratcliffe LE,Morgan SV,Simpson JE,Owens H,Vazquez-Villaseñor I,Heath PR,Romero IA,Ince PG,Wharton SB

44-550G was used in western blot to study the insulin/IGF1 signaling pathway in human astrocytes.

Sat Aug 22 00:00:00 UTC 2015

Abnormal serine phosphorylation of insulin receptor substrate 1 is associated with tau pathology in Alzheimer's disease and tauopathies.

Acta neuropathologica

Yarchoan M,Toledo JB,Lee EB,Arvanitakis Z,Kazi H,Han LY,Louneva N,Lee VM,Kim SF,Trojanowski JQ,Arnold SE

44-550G was used in immunohistochemistry - paraffin section to determine the expression of abnormal serine phosphorylation of insulin receptor substrate 1 in healthy and diseased human brains

Sat Nov 01 00:00:00 UTC 2014

High fat diet produces brain insulin resistance, synaptodendritic abnormalities and altered behavior in mice.

Neurobiology of disease

Arnold SE,Lucki I,Brookshire BR,Carlson GC,Browne CA,Kazi H,Bang S,Choi BR,Chen Y,McMullen MF,Kim SF

44-550G was used in immunohistochemistry - paraffin section to determine how insulin resistance contributes to cognitive health.

Tue Jul 01 00:00:00 UTC 2014

High fat diet produces brain insulin resistance, synaptodendritic abnormalities and altered behavior in mice.

Neurobiology of disease

Arnold SE,Lucki I,Brookshire BR,Carlson GC,Browne CA,Kazi H,Bang S,Choi BR,Chen Y,McMullen MF,Kim SF

44-550G was used in immunohistochemistry - paraffin section to determine how insulin resistance contributes to cognitive health.

Tue Jul 01 00:00:00 UTC 2014

High fat diet produces brain insulin resistance, synaptodendritic abnormalities and altered behavior in mice.

Neurobiology of disease

Arnold SE,Lucki I,Brookshire BR,Carlson GC,Browne CA,Kazi H,Bang S,Choi BR,Chen Y,McMullen MF,Kim SF

44-550G was used in immunohistochemistry - paraffin section to determine how insulin resistance contributes to cognitive health.

Tue Jul 01 00:00:00 UTC 2014

Inhibition of FAK signaling activated by urokinase receptor induces dormancy in human carcinoma cells in vivo.

Oncogene

Aguirre Ghiso JA

Thu Apr 11 00:00:00 UTC 2002

Bioinformatics

Protein Aliases: Insulin receptor substrate 1; IRS-1; pp185

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Gene Aliases: G972R; HIRS-1; IRS-1; IRS1; IRS1IRM

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UniProt ID: (Human) P35568, (Rat) P35570, (Mouse) P35569

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Entrez Gene ID: (Human) 3667, (Rat) 25467, (Mouse) 16367

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Function(s)

signal transducer activity transmembrane receptor protein tyrosine kinase adaptor activity protein kinase C binding Ras guanyl-nucleotide exchange factor activity insulin receptor binding insulin-like growth factor receptor binding protein binding 1-phosphatidylinositol-3-kinase activity SH2 domain binding phosphatidylinositol 3-kinase binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein kinase binding protein domain specific binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-IRS1 (Ser616) Polyclonal Antibody

Phospho-IRS1 (Ser616) Antibody (44-550G) in IHC (P)

Product Details

44-550G

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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