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Phospho-IkappaB epsilon (Ser161) Antibody (PA5-104688) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-IkappaB epsilon (Ser22) in human appendiceal tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-IkappaB epsilon (Ser22) polyclonal antibody (Product # PA5-104688) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
产品信息
PA5-104688
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human NFKBIE(Accession O00221), corresponding to amino acid residues around phosphorylated Ser161.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
sequential chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2816161
产品详细信息
Antibody detects endogenous levels of IKB epsilon only when phosphorylated at Serine 161.
靶标信息
NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine). For some genes, activation requires NFKB interaction with other transcription factors, such as STAT (see STAT6, MIM 601512), AP1 (JUN, MIM 165160), and NFAT (see NFATC1, MIM 600489).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: I-kappa-B-epsilon; ikappaBepsilon; IkB epsilon; IkB-E; ikB-epsilon; IkBepsilon; NF-kappa-B inhibitor epsilon; NF-kappa-BIE; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon; solute carrier family 35, member B2
基因别名: I-kappa-B-epsilon; IkB-E; ikB-epsilon; IKBE; NF-kappa-BIE; NFKBIE; Slc35b2
UniProt ID: (Human) O00221, (Mouse) O54910
Entrez Gene ID: (Human) 4794, (Mouse) 18037, (Rat) 316241
