Invitrogen

Phospho-KCC2 (Ser940) Polyclonal Antibody

Phospho-KCC2 (Ser940) Antibody in Western Blot (WB)

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Phospho-KCC2 (Ser940) Antibody (PA5-95678) in WB

Western blot of KCC2 in rat hippocampal lysate showing specific labeling of a band at ~135 kDa corresponding to Phospho-KCC2 (Ser940) polyclonal antibody (Product # PA5-95678) in the first lane (-). Immunolabeling is blocked by pre-adsorption with the phosphopeptide used as antigen in the second lane (+), but not by the corresponding non-phosphopeptide (not shown).

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产品信息

PA5-95678

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫沉淀 (IP)

40 µL of antibody per 1mg lysate

产品规格

种属反应

Mouse, Rat

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser940 conjugated to KLH

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

~0.25 mg/mL

纯化类型

sequential chromatography

保存液

10mM HEPES, pH 7.5, with 100µg/mL BSA, 50% glycerol, 0.15M NaCl

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2807480

产品详细信息

Antigen Affinity Purified from Pooled Serum

靶标信息

Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Daily rhythm in cortical chloride homeostasis underpins functional changes in visual cortex excitability.

Nature communications

Pracucci E,Graham RT,Alberio L,Nardi G,Cozzolino O,Pillai V,Pasquini G,Saieva L,Walsh D,Landi S,Zhang J,Trevelyan AJ,Ratto GM

PA5-95678 was used in Western Blot to demonstrate a daily [Cl-]i modulation with complex effects on cortical excitability.

Sat Nov 04 00:00:00 UTC 2023

Daily rhythm in cortical chloride homeostasis underpins functional changes in visual cortex excitability.

Nature communications

Pracucci E,Graham RT,Alberio L,Nardi G,Cozzolino O,Pillai V,Pasquini G,Saieva L,Walsh D,Landi S,Zhang J,Trevelyan AJ,Ratto GM

PA5-95678 was used in Western Blot to demonstrate a daily [Cl-]i modulation with complex effects on cortical excitability.

Sat Nov 04 00:00:00 UTC 2023

The Chloride Homeostasis of CA3 Hippocampal Neurons Is Not Altered in Fully Symptomatic Mepc2-null Mice.

Frontiers in cellular neuroscience

Belaïdouni Y,Diabira D,Zhang J,Graziano JC,Bader F,Montheil A,Menuet C,Wayman GA,Gaiarsa JL

PA5-95678 was used in Western Blotting to assess KCC2 and NKCC1 expressions and chloride homeostasis in the hippocampus of early [postnatal (P) day 30-35] and late (P50-60) symptomatic male Mecp2-null (Mecp2 -/y) mice.

Tue May 17 00:00:00 UTC 2022

The Chloride Homeostasis of CA3 Hippocampal Neurons Is Not Altered in Fully Symptomatic Mepc2-null Mice.

Frontiers in cellular neuroscience

Belaïdouni Y,Diabira D,Zhang J,Graziano JC,Bader F,Montheil A,Menuet C,Wayman GA,Gaiarsa JL

Tue May 17 00:00:00 UTC 2022

Staurosporine and NEM mainly impair WNK-SPAK/OSR1 mediated phosphorylation of KCC2 and NKCC1.

PloS one

Zhang J,Cordshagen A,Medina I,Nothwang HG,Wisniewski JR,Winklhofer M,Hartmann AM

PA5-95678 was used in Western Blotting to investigate the regulatory mechanism of Staurosporine (broad kinase inhibitor) and N-ethylmalemide (NEM) on KCC2 and NKCC1.

Fri Aug 07 00:00:00 UTC 2020

Staurosporine and NEM mainly impair WNK-SPAK/OSR1 mediated phosphorylation of KCC2 and NKCC1.

PloS one

Zhang J,Cordshagen A,Medina I,Nothwang HG,Wisniewski JR,Winklhofer M,Hartmann AM

PA5-95678 was used in Western Blotting to investigate the regulatory mechanism of Staurosporine (broad kinase inhibitor) and N-ethylmalemide (NEM) on KCC2 and NKCC1.

Fri Aug 07 00:00:00 UTC 2020

生物信息学

蛋白别名: Electroneutral potassium-chloride cotransporter 2; Furosemide-sensitive K-Cl cotransporter; K-Cl cotransporter 2; KCC 2; KCC2a variant 1; KCC2a-S25 variant 1; KCC2b variant 2; mKCC2; Neuronal K-Cl cotransporter; neuronal-specific K-Cl cotransporter; rKCC2; S12A5; Solute carrier family 12 member 5; solute carrier family 12, member 5

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基因别名: KCC2; Kiaa1176; mKIAA1176; Slc12a5

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UniProt ID: (Mouse) A2A5L0, (Rat) Q63633

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Entrez Gene ID: (Mouse) 57138, (Rat) 171373

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功能

protein binding ammonium transmembrane transporter activity symporter activity cation:chloride symporter activity potassium:chloride symporter activity protein kinase binding secondary carrier transporter

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