Invitrogen

Phospho-KSR2 (Thr497) Polyclonal Antibody

Phospho-KSR2 (Thr497) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Phospho-KSR2 (Thr497) Antibody (PA5-105093) in IHC (P)

Immunohistochemistry analysis of Phospho-KSR2 (Thr497) in rat colorectal tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Phospho-KSR2 (Thr497) polyclonal antibody (Product # PA5-105093) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

Phospho-KSR2 (Thr497) Antibody in Western Blot (WB)

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产品信息

PA5-105093

应用

建议稀释比

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免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human KSR2(Accession Q6VAB6), corresponding to amino acid residues around phosphorylated Thr497.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

sequential chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2816566

产品详细信息

Antibody detects endogenous levels of KSR2 only when phosphorylated at Thr497.

靶标信息

KSR2 serine/threonine kinase or kinase suppressor of Ras 2 specifically regulates the activity of MEKK3 and COT but not other members of the MAP3K family. KSR interacts with a regulatory Raf molecule in cis to induce a conformational switch of MEK, facilitating MEK's phosphorylation by a separate catalytic Raf molecule in trans. Blocks MAP3K8 kinase activity and MAP3K8- mediated signaling. Acts as a negative regulator of MAP3K3- mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.

仅用于科研。不用于诊断过程。未经明确授权不得转售。