Invitrogen

Phospho-LAT (Tyr132) Polyclonal Antibody

Phospho-LAT (Tyr132) Antibody in Western Blot (WB)

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Phospho-LAT (Tyr132) Antibody (44-224) in WB

Peptide Competition: Extracts prepared from Jurkat E6.1 cells were left unstimulated (1) or stimulated (2-5), resolved by SDS-PAGE on a 10% polyacrylamide gel, and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C.

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产品信息

44-224

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫细胞化学 (ICC/IF)

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human LAT that contains tyrosine 132.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533608

靶标信息

LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A single-amino acid substitution in the adaptor LAT accelerates TCR proofreading kinetics and alters T-cell selection, maintenance and function.

Nature immunology

Lo WL,Kuhlmann M,Rizzuto G,Ekiz HA,Kolawole EM,Revelo MP,Andargachew R,Li Z,Tsai YL,Marson A,Evavold BD,Zehn D,Weiss A

44-224 was used in Western Blot to reveal the importance of kinetic proofreading in balancing tolerance and immunity.

Sat Apr 01 00:00:00 UTC 2023

Aging-associated HELIOS deficiency in naive CD4⁺ T cells alters chromatin remodeling and promotes effector cell responses.

Nature immunology

Zhang H,Jadhav RR,Cao W,Goronzy IN,Zhao TV,Jin J,Ohtsuki S,Hu Z,Morales J,Greenleaf WJ,Weyand CM,Goronzy JJ

44-224 was used in Western Blotting to identify increased phosphorylation of STAT5, at least in part due to aberrant IL-2 receptor and lower HELIOS expression, as upstream regulators.

Sun Jan 01 00:00:00 UTC 2023

Kinetic proofreading through the multi-step activation of the ZAP70 kinase underlies early T cell ligand discrimination.

Nature immunology

Voisinne G,Locard-Paulet M,Froment C,Maturin E,Menoita MG,Girard L,Mellado V,Burlet-Schiltz O,Malissen B,Gonzalez de Peredo A,Roncagalli R

44-224 was used in Western Blotting to provide a comprehensive map of molecular events associated with T cell ligand discrimination.

Thu Sep 01 00:00:00 UTC 2022

PLCγ1 promotes phase separation of T cell signaling components.

The Journal of cell biology

Zeng L,Palaia I,Šarić A,Su X

44-224 was used in Western Blot to report that phospholipase Cγ1 (PLCγ1) promotes phase separation of LAT, a key adaptor protein in the T cell receptor pathway.

Mon Jun 07 00:00:00 UTC 2021

PLCγ1 promotes phase separation of T cell signaling components.

The Journal of cell biology

Zeng L,Palaia I,Šarić A,Su X

44-224 was used in Western Blot to report that phospholipase Cγ1 (PLCγ1) promotes phase separation of LAT, a key adaptor protein in the T cell receptor pathway.

Mon Jun 07 00:00:00 UTC 2021

The Cish SH2 domain is essential for PLC-γ1 regulation in TCR stimulated CD8⁺ T cells.

Scientific reports

Guittard G,Dios-Esponera A,Palmer DC,Akpan I,Barr VA,Manna A,Restifo NP,Samelson LE

44-224 was used in Immunocytochemistry to study the structural and functional relationships between Crish and PLC-γ1 during CD8+ T cell activation.

Wed Mar 28 00:00:00 UTC 2018

LFA-1 activates focal adhesion kinases FAK1/PYK2 to generate LAT-GRB2-SKAP1 complexes that terminate T-cell conjugate formation.

Nature communications

Raab M,Lu Y,Kohler K,Smith X,Strebhardt K,Rudd CE

Published figure using Phospho-LAT (Tyr132) polyclonal antibody (Product # 44-224) in Western Blot

Wed Jul 12 00:00:00 UTC 2017

Small molecule inhibition of Csk alters affinity recognition by T cells.

eLife

Manz BN,Tan YX,Courtney AH,Rutaganira F,Palmer E,Shokat KM,Weiss A

Published figure using Phospho-LAT (Tyr132) polyclonal antibody (Product # 44-224) in Western Blot

Mon Aug 24 00:00:00 UTC 2015

Inhibition of the kinase Csk in thymocytes reveals a requirement for actin remodeling in the initiation of full TCR signaling.

Nature immunology

Tan YX,Manz BN,Freedman TS,Zhang C,Shokat KM,Weiss A

Published figure using Phospho-LAT (Tyr132) polyclonal antibody (Product # 44-224) in Western Blot

Sat Feb 01 00:00:00 UTC 2014

Quantitative and temporal requirements revealed for Zap70 catalytic activity during T cell development.

Nature immunology

Au-Yeung BB,Melichar HJ,Ross JO,Cheng DA,Zikherman J,Shokat KM,Robey EA,Weiss A

44-224 was used in western blot to examine the quantitative and temporal requirements of Zap70's function in thymocyte development

Tue Jul 01 00:00:00 UTC 2014

Stability of an autoinhibitory interface in the structure of the tyrosine kinase ZAP-70 impacts T cell receptor response.

Proceedings of the National Academy of Sciences of the United States of America

Deindl S,Kadlecek TA,Cao X,Kuriyan J,Weiss A

Tue Dec 08 00:00:00 UTC 2009

Intramolecular regulatory switch in ZAP-70: analogy with receptor tyrosine kinases.

Molecular and cellular biology

Brdicka T,Kadlecek TA,Roose JP,Pastuszak AW,Weiss A

Wed Jun 01 00:00:00 UTC 2005

生物信息学

蛋白别名: 36 kDa phospho-tyrosine adapter protein; 36 kDa phospho-tyrosine adaptor protein; 36 kDa phosphotyrosine adapter protein; linker for activation of T cells; linker for activation of T cells, transmembrane adaptor; Linker for activation of T-cells family member 1; p36-38; pp36

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基因别名: LAT; LAT1; pp36

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UniProt ID: (Human) O43561

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Entrez Gene ID: (Human) 27040

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功能

SH3/SH2 adaptor activity Ras guanyl-nucleotide exchange factor activity protein binding protein kinase binding

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