Invitrogen

Phospho-MAPKAPK2 (Thr334) Recombinant Rabbit Monoclonal Antibody (MAPKAPK2T334-H2)

Phospho-MAPKAPK2 (Thr334) Antibody in Western Blot (WB)

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Phospho-MAPKAPK2 (Thr334) Antibody (MA5-37010) in WB

Western blot analysis of Phospho-MAPKAPK2 (Thr334) using HeLa cell extract. Samples were untreated or treated with UV and TPA, and incubated in monoclonal Phospho-MAPKAPK2 (Thr334) antibody (Product # MA5-37010) with a dilution of 0.05 µg/mL.

Phospho-MAPKAPK2 (Thr334) Antibody in Flow Cytometry (Flow)

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产品信息

MA5-37010

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

流式细胞分析 (Flow)

1-1,000 ng/mL

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG, kappa

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

MAPKAPK2T334-H2

抗原

A synthetic phospho-peptide corresponding to residues surrounding Thr334 of human phospho MAPKAPK-2.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A/G

保存液

PBS, pH 7.4, with 50% glycerol, 0.1% BSA

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2896945

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

MAPKAPK2 mediates p38 and ERK signaling in vivo. It is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation, and cell proliferation. MAPKAPK2 encodes a member of the Ser/Thr protein kinase family. MAPKAPK2 is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, MAPKAPK2 is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for MAPKAPK2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。