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Phospho-MARK2 (Thr596) Antibody (PA5-105815) in ICC/IF
Immunofluorescent analysis of Phospho-MARK2 (Thr596) in HeLa cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-MARK2 (Thr596) polyclonal antibody (Product # PA5-105815) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 59... View More
产品信息
PA5-105815
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human MARK2(Accession Q7KZI7), corresponding to amino acid residues around phosphorylated Thr596.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
sequential chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2817214
产品详细信息
Antibody detects endogenous levels of MARK2 only when phosphorylated at Thr596.
靶标信息
MARK2 is a serine/threonine protein kinase involved in the control of cell polarity and microtubule stability. Several cDNA clones have been isolated that encoded two isoforms of the human ser/thr protein kinase EMK1. These isoforms were characterized by the presence of a 162-bp alternative exon that gave rise to two forms, one containing the exon and the other one lacking it. Both forms were found to be coexpressed in a number of selected cell lines and tissue samples. The human EMK1 was shown to be encoded by a single mRNA ubiquitously expressed.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ELKL motif kinase; ELKL motif kinase 1; EMK-1; EMK1; MAP/microtubule affinity-regulating kinase 2; MARK2; mark2 {ECO:0000312|RGD:708483}; MGC99619; OTTHUMP00000218338; OTTHUMP00000218339; OTTHUMP00000218340; OTTHUMP00000218343; Par-1b; PAR1 homolog; PAR1 homolog b; Ser/Thr protein kinase PAR-1B; serine/threonine kinase; serine/threonine protein kinase EMK; Serine/threonine-protein kinase MARK2; testicular tissue protein Li 117
基因别名: Emk; EMK-1; EMK1; MARK2; PAR-1; Par-1b; Par1b
UniProt ID: (Human) Q7KZI7, (Rat) O08679, (Mouse) Q05512
Entrez Gene ID: (Human) 2011, (Rat) 60328, (Mouse) 13728
magnesium ion binding
protein serine/threonine kinase activity
protein binding
ATP binding
lipid binding
protein kinase activator activity
poly(A) RNA binding
tau-protein kinase activity
cadherin binding involved in cell-cell adhesion
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
metal ion binding
non-receptor serine/threonine protein kinase
microtubule cytoskeleton organization
mitophagy
neuron migration
protein phosphorylation
positive regulation of neuron projection development
Wnt signaling pathway
peptidyl-threonine phosphorylation
establishment of cell polarity
activation of protein kinase activity
intracellular signal transduction
establishment or maintenance of epithelial cell apical/basal polarity
protein autophosphorylation
regulation of axonogenesis
regulation of cytoskeleton organization
mitochondrion localization
cell-cell adhesion
multicellular organism development
phosphorylation
cell differentiation
