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Phospho-MKP-1 (Ser323) Antibody (PA5-106010) in ICC/IF
Immunocytochemistry analysis of Phospho-MKP-1 (Ser323) in A549 cells (H2O2 treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-MKP-1 (Ser323) polyclonal antibody (Product # PA5-106010) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (... View More
产品信息
PA5-106010
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human DUSP1(Accession P28562), corresponding to amino acid residues around phosphorylated Ser323.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
sequential chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2817409
产品详细信息
Antibody detects endogenous levels of DUSP1/MKP1 only when phosphorylated at Ser323.
靶标信息
The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 3CH134/CL100 PTPase; CL 100; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; DUS1; EC 3.1.3.16; EC 3.1.3.48; MAP kinase phosphatase 1; MAP kinase phosphatase-1; mitogen-activated protein (MAP) kinase phosphatase-1; Mitogen-activated protein kinase phosphatase 1; mitogen-activated protein kinase phosphatase-1; oxidative stress-inducible protein tyrosine phosphatase; protein tyrosine phosphatase non-receptor type 16; protein tyrosine phosphatase, non-receptor type 16; Protein-tyrosine phosphatase 3CH134; Protein-tyrosine phosphatase CL100; Protein-tyrosine phosphatase ERP; Protein-tyrosine phosphatase non-receptor type 16; serine/threonine specific protein phosphatase
基因别名: 3CH134; CL100; DUSP1; erp; HVH1; MKP-1; MKP1; PTPN10; Ptpn16; U19515; VH1
UniProt ID: (Human) P28562, (Rat) Q64623, (Mouse) P28563
Entrez Gene ID: (Human) 1843, (Rat) 114856, (Mouse) 19252
non-membrane spanning protein tyrosine phosphatase activity
protein binding
protein tyrosine/threonine phosphatase activity
MAP kinase tyrosine/serine/threonine phosphatase activity
growth factor binding
protein tyrosine phosphatase activity
protein tyrosine/serine/threonine phosphatase activity
phosphoprotein phosphatase activity
hydrolase activity
phosphatase activity
protein phosphatase
inactivation of MAPK activity
endoderm formation
response to oxidative stress
response to light stimulus
response to estradiol
response to retinoic acid
cellular response to hormone stimulus
response to testosterone
peptidyl-tyrosine dephosphorylation
intracellular signal transduction
peptidyl-threonine dephosphorylation
response to hydrogen peroxide
regulation of apoptotic process
positive regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of MAP kinase activity
negative regulation of MAPK cascade
response to glucocorticoid
negative regulation of meiotic cell cycle
response to cAMP
response to calcium ion
negative regulation of ERK1 and ERK2 cascade
mitotic cell cycle arrest
regulation of mitotic cell cycle spindle assembly checkpoint
negative regulation of DNA biosynthetic process
protein dephosphorylation
response to organic substance
cell cycle
dephosphorylation
