Invitrogen

Phospho-MKP1/MKP2 (Ser296, Ser318) Polyclonal Antibody

Phospho-MKP1/MKP2 (Ser296, Ser318) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-MKP1/MKP2 (Ser296, Ser318) Antibody (PA5-104854) in ICC/IF

Immunofluorescent analysis of Phospho-MKP1/MKP2 (Ser296, Ser318) in HeLa cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-MKP1/MKP2 (Ser296, Ser318) polyclonal antibody (Product # PA5-104854) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-r... View More

Phospho-MKP1/MKP2 (Ser296, Ser318) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-MKP1/MKP2 (Ser296, Ser318) Antibody in Western Blot (WB)

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产品信息

PA5-104854

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human DUSP1(Accession P28562), corresponding to amino acid residues around phosphorylated Ser296.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

sequential chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2816327

产品详细信息

Antibody detects endogenous levels of MKP1/2 only when phosphorylated at Serine 296/318.

靶标信息

There are ten mitogen-activated protein kinase (MAPK) phosphatases (MKPs) that act as negative regulators of MAPK activity in mammalian cells. MKP2 (DUSP4) is a dual threonine/tyrosine phosphatase that specifically dephosphorylates and inactivates MAPKs (ERK, JNK, p38). It contains one Rhodanese domain and one tyrosine-protein phosphatase domain. MKP2 is expressed in a variety of tissues, and is localized in the nucleus. Transcription factor E2F-1 acts as a transcriptional regulator of MKP2. E2F-1/MKP2 pathway mediates apoptosis under oxidative stress and that MKP2 suppresses tumor formation. MKP2 is a novel transcription target of p53 in mediating apoptosis by 10-bp palindrome motif (CTGGCGCCAG) in the MKP2 promoter.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 3CH134/CL100 PTPase; CL 100; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase 4; Dual specificity protein phosphatase hVH1; Dual specificity protein phosphatase hVH2; DUSP 4; DUSP-4; MAP kinase phosphatase 1; MAP kinase phosphatase 2; MAP kinase phosphatase-1; mitogen-activated protein (MAP) kinase phosphatase-1; Mitogen-activated protein kinase phosphatase 1; Mitogen-activated protein kinase phosphatase 2; mitogen-activated protein kinase phosphatase-1; MKP II; MKPII; oxidative stress-inducible protein tyrosine phosphatase; protein tyrosine phosphatase non-receptor type 16; protein tyrosine phosphatase, non-receptor type 16; Protein-tyrosine phosphatase 3CH134; Protein-tyrosine phosphatase CL100; Protein-tyrosine phosphatase ERP; Protein-tyrosine phosphatase non-receptor type 16; serine/threonine specific protein phosphatase; VH1 homologous phosphatase 2

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基因别名: 2700078F24Rik; 3CH134; AI844617; BB104621; CL100; DUSP1; DUSP4; E130306H24Rik; erp; HVH1; HVH2; MKP-1; MKP-2; MKP1; MKP2; PTPN10; Ptpn16; TYP; U19515; VH1; VH2