Invitrogen

Phospho-MYL2 (Ser15) Polyclonal Antibody

Phospho-MYL2 (Ser15) Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 16

Phospho-MYL2 (Ser15) Antibody (PA5-104265) in ICC/IF

Immunofluorescent analysis of Phospho-MYL2 (Ser15) in HepG2 during mitosis. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-MYL2 (Ser15) polyclonal antibody (Product # PA5-104265) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goa... View More

Phospho-MYL2 (Ser15) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-MYL2 (Ser15) Antibody in Western Blot (WB)

Product Image Gallery

产品信息

PA5-104265

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:200-1:2,000

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human MYL2(Accession P10916), corresponding to amino acid residues around phosphorylated Ser15.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

sequential chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2816014

产品详细信息

Antibody detects endogenous levels of MLC2 only when phosphorylated at Ser15.

靶标信息

Thus gene encodes the regulatory light chain associated with cardiac myosin beta heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Phosphatase regulatory subunit MYPT2 knockout partially compensates for the cardiac dysfunction in mice caused by lack of myosin light chain kinase 3.

The Journal of biological chemistry

Hu T,Kalyanaraman H,Pilz RB,Casteel DE

Published figure using Phospho-MYL2 (Ser15) polyclonal antibody (Product # PA5-104265) in Western Blot

Sat Apr 01 00:00:00 UTC 2023

Loss of cardiac myosin light chain kinase contributes to contractile dysfunction in right ventricular pressure overload.

Physiological reports

Prasad V,Makkaoui N,Rajan R,Patel A,Mainali B,Bagchi P,Kumar R,Rogers J,Diamond J,Maxwell JT

PA5-104265 was used in Western Blotting to evaluate the adaptive mechanisms of congenital heart defect patients in juvenile rats.

Fri Apr 01 00:00:00 UTC 2022

Integrated multi-omics analysis of adverse cardiac remodeling and metabolic inflexibility upon ErbB2 and ERRα deficiency.

Communications biology

Dufour CR,Xia H,B'chir W,Perry MC,Kuzmanov U,Gainullina A,Dejgaard K,Scholtes C,Ouellet C,Zuo D,Sanguin-Gendreau V,Guluzian C,Smith HW,Muller WJ,Audet-Walsh E,Sergushichev AA,Emili A,Giguère V

PA5-104265 was used in Immuno-assay to reveal potential cardiovascular risks in discrete combination therapies in the treatment of breast and other cancers.

Mon Sep 12 00:00:00 UTC 2022

生物信息学

蛋白别名: Cardiac myosin light chain 2; cardiac myosin light chain-2; cardiac ventricular myosin light chain 2; DKFZp779C0562; MLC-2; MLC-2s/v; MLC-2v; Myosin light chain 2, slow skeletal/ventricular muscle isoform; myosin light chain 2v; myosin light chain phosphatase; myosin light chain, phosphorylatable, cardiac ventricles; myosin light chain-2; Myosin regulatory light chain 2, ventricular/cardiac muscle isoform; myosin, light chain 2, regulatory, cardiac, slow; myosin, light polypeptide 2, regulatory, cardiac, slow; regulatory light chain of myosin; RLC of myosin; slow cardiac myosin regulatory light chain 2; Ventricular myosin light chain 2; ventricular myosin regulatory light chain

查看更多收起

基因别名: CMH10; MLC-2; MLC-2s/v; MLC-2v; MLC2; Mlc2v; MYL2; Mylpc

查看更多收起

UniProt ID: (Human) P10916, (Rat) P08733, (Mouse) P51667

查看更多收起

Entrez Gene ID: (Human) 4633, (Rat) 363925, (Mouse) 17906

查看更多收起

功能

actin monomer binding calcium ion binding protein binding structural constituent of muscle myosin heavy chain binding metal ion binding actin or actin-binding cytoskeletal protein

参与通路