Invitrogen

Phospho-MYL9 (Ser19) Monoclonal Antibody (R.179.1)

Phospho-MYL9 (Ser19) Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 3

Phospho-MYL9 (Ser19) Antibody (MA5-15163) in ICC/IF

Immunofluorescent analysis of Phospho-Myosin Light Chain 2 pSer19 in HeLa cells using a Phospho-Myosin Light Chain 2 pSer19 onoclonal antibody (Product # MA5-15163) (green). Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.

Phospho-MYL9 (Ser19) Antibody in Western Blot (WB)

Product Image Gallery

产品信息

MA5-15163

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

1:200-1:400

查看4篇已发表文章 4篇已发表文章

产品规格

种属反应

Bovine, Human, Mouse, Pig, Rat

已发表种属

Human, Mouse, Rat, Xenopus

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

R.179.1

抗原

Synthetic phosphopeptide corresponding to residues surrounding pSer19 of human myosine light chain 2 (smooth muscle)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

445 µg/mL

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

<0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_10979087

产品详细信息

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with the cardiac isoform of myosin light chain 2.

靶标信息

Myosin, a structural component of muscle, consists of two heavy chains and four light chains. Myosin light chain may regulate muscle contraction by modulating the ATPase activity of myosin heads. It also binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Unique osteogenic profile of bone marrow stem cells stimulated in perfusion bioreactor is Rho-ROCK-mediated contractility dependent.

Bioengineering & translational medicine

Yamada S,Yassin MA,Torelli F,Hansmann J,Green JBA,Schwarz T,Mustafa K

MA5-15163 was used in Immunohistochemistry to analyse the cytoskeletal modulation and osteogenic profiles of BMSC under fluid stimuli in a 3D culture condition using a perfusion bioreactor.

Mon May 01 00:00:00 UTC 2023

Switching between blebbing and lamellipodia depends on the degree of non-muscle myosin II activity.

Journal of cell science

Ghosh I,Singh RK,Mishra M,Kapoor S,Jana SS

MA5-15163 was used in Immunocytochemistry to demonstrate how ROCK and MLCK, and subsequently non-muscle myosin II activity, contribute to either blebbing or lamellipodia modes of cellular migration.

Wed Jan 13 00:00:00 UTC 2021

Behind Brain Metastases Formation: Cellular and Molecular Alterations and Blood-Brain Barrier Disruption.

International journal of molecular sciences

Godinho-Pereira J,Garcia AR,Figueira I,Malhó R,Brito MA

MA5-15163 was used in Immunocytochemistry to study the components involved in the interaction between breast cancer cells and blood-brain barrier (BBB) endothelial cells underlying BBB alterations and the transendothelial migration of malignant cells.

Wed Jun 30 00:00:00 UTC 2021

ADF and cofilin-1 collaborate to promote cortical actin flow and the leader bleb-based migration of confined cells.

eLife

Ullo MF,Logue JS

MA5-15163 was used in Immunocytochemistry to identify cofilin-1 and ADF as actin remodeling factors required for fast amoeboid migration.

Fri Jun 25 00:00:00 UTC 2021

Doxorubicin induces detrusor smooth muscle impairments through myosin dysregulation, leading to a risk of lower urinary tract dysfunction.

American journal of physiology. Renal physiology

Iguchi N,Dönmez Mİ,Carrasco A,Wilcox DT,Pineda RH,Malykhina AP,Cost NG

MA5-15163 was used in Western Blot, Immunocytochemistry to show that sensing of endogenous nucleic acids by ZBP1 is critical in driving skin pathology characterised by antiviral and IL-17 immune responses.

Mon Jul 01 00:00:00 UTC 2019

Doxorubicin induces detrusor smooth muscle impairments through myosin dysregulation, leading to a risk of lower urinary tract dysfunction.

American journal of physiology. Renal physiology

Iguchi N,Dönmez Mİ,Carrasco A,Wilcox DT,Pineda RH,Malykhina AP,Cost NG

Mon Jul 01 00:00:00 UTC 2019

Septin-dependent remodeling of cortical microtubule drives cell reshaping during epithelial wound healing.

Journal of cell science

Shindo A,Audrey A,Takagishi M,Takahashi M,Wallingford JB,Kinoshita M

MA5-15163 was used in Immunohistochemistry-immunofluorescence to demonstrate the role of septins in reorganizing microtubules and remodeling surrounding cell shape during embryonic wound closure.

Thu Jun 28 00:00:00 UTC 2018

生物信息学

蛋白别名: 20 kDa myosin light chain; LC20; MGC3505; MLC-2C; myosin light chain, regulatory C; myosin regulatory light chain; myosin regulatory light chain 1; Myosin regulatory light chain 2, smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRLC1; Myosin regulatory light polypeptide 9; Myosin RLC; myosin, light chain 9, regulatory; myosin, light polypeptide 9, regulatory

查看更多收起

基因别名: AI327049; LC20; MLC-2C; MLC2; MLC20; MRLC1; MYL9; Mylc2c; MYRL2; RLC-C