Invitrogen

Phospho-MYPT1/MYPT2 (Ser668, Ser618) Polyclonal Antibody

Phospho-MYPT1/MYPT2 (Ser668, Ser618) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Phospho-MYPT1/MYPT2 (Ser668, Ser618) Antibody (PA5-118764) in IHC (P)

Immunohistochemical analysis of Phospho-MYPT1/MYPT2 (Ser668, Ser618) in Rat testis tissue using a Phospho-MYPT1/MYPT2 (Ser668, Ser618) polyclonal antibody (Product # PA5-118764). The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antib... View More

Phospho-MYPT1/MYPT2 (Ser668, Ser618) Antibody in Western Blot (WB)

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产品信息

PA5-118764

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human PPP1R12A(Accession O14974), corresponding to amino acid residues around phosphorylated Ser668.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

sequential chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2903264

产品详细信息

Antibody detects endogenous levels of MYPT1/2 only when phosphorylated at Ser668/Ser618.

靶标信息

Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: hHS-M21A; MBSP; myosin binding subunit; Myosin phosphatase target subunit 1; Myosin phosphatase target subunit 2; myosin phosphatase, target subunit 1; myosin phosphatase, target subunit 2; Myosin phosphatase-targeting subunit 1; Myosin phosphatase-targeting subunit 2; PP-1M; Protein phosphatase 1 regulatory subunit 12A; Protein phosphatase 1 regulatory subunit 12B; protein phosphatase 1, regulatory (inhibitor) subunit 12A; protein phosphatase 1, regulatory (inhibitor) subunit 12B; protein phosphatase 1, regulatory subunit 12A; protein phosphatase 1, regulatory subunit 12B; Protein phosphatase myosin-binding subunit; Protein phosphatase subunit 1M; Serine/threonine protein phosphatase PP1 smooth muscle regulatory subunit M110; smooth muscle myosin phosphatase myosin-binding subunit

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基因别名: 1200015F06Rik; 1810037O03Rik; 5730577I22Rik; 9530009M10Rik; AA792106; AI132431; AV099298; D10Ertd625e; M110; M130; MBS; MBSP; MYPT1; MYPT2; PP1bp55; PPP1R12A; PPP1R12B