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Phospho-NF2 (Ser518) Polyclonal Antibody

Phospho-NF2 (Ser518) Antibody in Western Blot (WB)

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Phospho-NF2 (Ser518) Antibody (600-401-414) in WB

Affinity purified phospho-specific antibody to NF2 (Merlin) at pS518 was used at a 1:1000 dilution to detect NF2 by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lane 1) or CLA treated at 4nM, 20 nM or 100 nM (lanes 2, 3 and 4 respectively) for 45. After washing, a 1:5,000 dilution of HRP conju... View More

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产品信息

600-401-414

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500- 1:2,000

免疫组化 (IHC)

Assay-dependent

酶联免疫吸附实验 (ELISA)

1:1,200,000

免疫沉淀 (IP)

1:500- 1:2,000

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Human NF2 (Merlin) phospho-peptide corresponding to a region of the human protein surrounding S518 and conjugated to Keyhole Limpet Hemocyanin (KLH).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Directed against human NF2 (Neurofibromatosis 2 gene product). This phospho-specific polyclonal antibody is specific for phosphorylated pS518 of human NF2 (neurofibromatosis 2 gene product). Reactivity with non-phosphorylated Human NF2 (Merlin) is minimal by ELISA. Cross-reactivity with NF2 (Merlin) occurs in mouse tissue. Reactivity with NF2 from other sources has not been determined.

靶标信息

NF2 encodes a protein, Merlin, that is similar to some members of the ERM family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. Merlin has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Merlin; MGC123124; moesin-ezrin-radixin like; Moesin-ezrin-radixin-like protein; moesin-ezrin-radizin-like protein; neurofibromin 2 (bilateral acoustic neuroma); neurofibromin 2 (merlin); Neurofibromin-2; OTTHUMP00000199607; Schwannomerlin; Schwannomin

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基因别名: ACN; BANF; merlin; Nf-2; NF2; SCH

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UniProt ID: (Human) P35240, (Mouse) P46662

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Entrez Gene ID: (Human) 4771, (Mouse) 18016

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功能

actin binding integrin binding protein binding beta-catenin binding protein domain specific binding cytoskeletal protein binding actin or actin-binding cytoskeletal protein

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