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Phospho-PAK1/PAK2 (Ser199, Ser204, Ser192, Ser197) Antibody (PA5-104628) in ICC/IF
Immunocytochemistry analysis of Phospho-PAK1/PAK2 (Ser199, Ser204, Ser192, Ser197) in Heat-shock treated Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-PAK1/PAK2 (Ser199, Ser204, Ser192, Ser197) polyclonal antibody (Product # PA5-104628) and mouse anti-beta tubulin antibody for 1 hour a... View More
产品信息
PA5-104628
应用
建议稀释比
已发表文章
产品规格
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human PAK1(Accession Q13153), corresponding to amino acid residues around phosphorylated Ser204.
偶联物
形式
Liquid
浓度
1 mg/mL
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2816103
产品详细信息
Antibody detectsAntibody detects endogenous levels of PAK1 when phosphorylated at Ser199/204 or PAK2 when phosphorylated at Ser192/197.
靶标信息
Three isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on gammaPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with gammaPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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