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Phospho-PKA alpha (Thr197) Antibody (PA5-121287) in WB
Western blot analysis of Phospho-PKA alpha (Thr197) in extracts of various cell lines. Samples were incubated with Phospho-PKA alpha (Thr197) Polyclonal antibody (Product # PA5-121287) using a dilution of 1:2,000 or PKA C-alpha (PRKACA) antibody (Product # MA5-37857). Followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. HeLa cells were treated by Calyculin A (100 nM) at 3... View More
产品信息
PA5-121287
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic phosphorylated peptide around T197 of human PKA C-alpha (PRKACA) (NP_002721.1).
偶联物
形式
Liquid
浓度
0.67 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2914859
产品详细信息
Positive test controls include: 293T, HeLa, C6. The target is usually found in the following locations: Cell membrane, Cell projection, Cytoplasm, Lipid-anchor, Membrane, Mitochondrion, Nucleus, cilium, flagellum.
Immunogen sequence: TWTLC
靶标信息
Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitous signaling pathway important in the cell cycle, cellular communication, memory formation and behavior. PKA is composed of two catalytic (PKAc; Protein Kinase A catalytic subunit) and two regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates to PKAr dimer and two activated PKAc ser/thr protein kinase catalytic monomers. The released PKAc can translocate into the nucleus and exert a regulatory role in the activation of multiple nuclear hormone receptors. However, PKAc-mediated activation of tonicity-dependent gene expression is cAMP independent. Humans express three types of PKAc subunit - PKAc alpha is present in most human tissues, PKAc beta and gamma are tissue-specific, the later is found in testes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: C(s); C-alpha-2; C-alpha-S; cAMP-dependent protein kinase catalytic subunit alpha; cAMP-dependent protein kinase catalytic subunit alpha, isoform 1; cAMP-dependent protein kinase catalytic subunit C alpha; MGC102831; MGC48865; PKA; PKA C-alpha; pkac; protein kinase A; protein kinase A catalytic subunit; protein kinase, cAMP-dependent, alpha catalytic subunit; protein kinase, cAMP-dependent, catalytic, alpha; sperm cAMP-dependent protein kinase catalytic subunit
基因别名: Cs-PKA; PKACA; PKCA1; PKCD; PPNAD4; PRKACA
UniProt ID: (Human) P17612, (Mouse) P05132
Entrez Gene ID: (Human) 5566, (Rat) 25636, (Mouse) 18747
protein serine/threonine kinase activity
cAMP-dependent protein kinase activity
protein serine/threonine/tyrosine kinase activity
protein binding
ATP binding
protein kinase binding
ubiquitin protein ligase binding
protein complex binding
protein kinase A regulatory subunit binding
protein kinase activity
Rab GTPase binding
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
G2/M transition of mitotic cell cycle
mesoderm formation
neural tube closure
regulation of heart rate
stimulatory C-type lectin receptor signaling pathway
renal water homeostasis
mRNA processing
protein phosphorylation
blood coagulation
positive regulation of cell proliferation
regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
activation of protein kinase A activity
calcium-mediated signaling using intracellular calcium source
lipoprotein metabolic process
regulation of protein binding
regulation of cellular respiration
regulation of osteoblast differentiation
protein autophosphorylation
positive regulation of protein export from nucleus
sperm capacitation
negative regulation of meiotic cell cycle
regulation of cytosolic calcium ion concentration
regulation of synaptic transmission, glutamatergic
regulation of cardiac muscle contraction
regulation of ryanodine-sensitive calcium-release channel activity
regulation of proteasomal protein catabolic process
regulation of protein processing
positive regulation of cell cycle arrest
cellular response to glucose stimulus
cellular response to parathyroid hormone stimulus
cellular response to glucagon stimulus
cellular response to epinephrine stimulus
cell communication by electrical coupling involved in cardiac conduction
negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning
regulation of cardiac conduction
regulation of bicellular tight junction assembly
modulation of synaptic transmission
phosphorylation
