Invitrogen

Phospho-PKA alpha/beta/gamma (Thr198) Polyclonal Antibody

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View all (3) PKA alpha/beta/gamma antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-105515

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
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Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human PRKACA(Accession P17612), corresponding to amino acid residues around phosphorylated Thr198.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2816943

Product Specific Information

Antibody detects endogenous levels of PKA alpha/beta/gamma CAT only when phosphorylated at Thr198.

Target Information

Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitous signaling pathway important in the cell cycle, cellular communication, memory formation and behavior. PKA is composed of two catalytic (PKAc; Protein Kinase A catalytic subunit) and two regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates to PKAr dimer and two activated PKAc ser/thr protein kinase catalytic monomers. The released PKAc can translocate into the nucleus and exert a regulatory role in the activation of multiple nuclear hormone receptors. However, PKAc-mediated activation of tonicity-dependent gene expression is cAMP independent. Humans express three types of PKAc subunit - PKAc alpha is present in most human tissues, PKAc beta and gamma are tissue-specific, the later is found in testes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: cAMP-dependent protein kinase C beta; cAMP-dependent protein kinase catalytic beta subunit isoform 4ab; cAMP-dependent protein kinase catalytic subunit alpha; cAMP-dependent protein kinase catalytic subunit alpha, isoform 1; cAMP-dependent protein kinase catalytic subunit beta; cAMP-dependent protein kinase catalytic subunit C alpha; cAMP-dependent protein kinase catalytic subunit gamma; PKA C-alpha; PKA C-beta; PKA C-gamma; protein kinase A; protein kinase A catalytic subunit; protein kinase A catalytic subunit beta; protein kinase, cAMP-dependent, alpha catalytic subunit; protein kinase, cAMP-dependent, beta catalytic subunit; protein kinase, cAMP-dependent, catalytic, alpha; protein kinase, cAMP-dependent, catalytic, beta; protein kinase, cAMP-dependent, catalytic, gamma; protein kinase, cAMP-dependent, gamma catalytic subunit; serine(threonine) protein kinase; sperm cAMP-dependent protein kinase catalytic subunit

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Gene Aliases: BDPLT19; Cs-PKA; KAPG; PKA C-beta; PKACA; PKACB; PKACg; PKCA1; PKCD; PPNAD4; PRKACA; PRKACB; PRKACG

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UniProt ID: (Human) P17612, (Human) P22694, (Human) P22612, (Mouse) P05132, (Rat) P68182, (Mouse) P68181

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Entrez Gene ID: (Human) 5566, (Human) 5567, (Human) 5568, (Rat) 25636, (Mouse) 18747, (Rat) 293508, (Mouse) 18749

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Function(s)
protein serine/threonine kinase activity cAMP-dependent protein kinase activity protein serine/threonine/tyrosine kinase activity protein binding ATP binding protein kinase binding ubiquitin protein ligase binding protein complex binding protein kinase A regulatory subunit binding magnesium ion binding kinase activity protein kinase activity Rab GTPase binding nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase
Process(es)
G2/M transition of mitotic cell cycle mesoderm formation neural tube closure regulation of heart rate stimulatory C-type lectin receptor signaling pathway renal water homeostasis mRNA processing protein phosphorylation blood coagulation positive regulation of cell proliferation regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion peptidyl-serine phosphorylation peptidyl-threonine phosphorylation activation of protein kinase A activity calcium-mediated signaling using intracellular calcium source lipoprotein metabolic process regulation of protein binding regulation of cellular respiration regulation of osteoblast differentiation protein autophosphorylation positive regulation of protein export from nucleus sperm capacitation negative regulation of meiotic cell cycle regulation of cytosolic calcium ion concentration regulation of synaptic transmission, glutamatergic regulation of cardiac muscle contraction regulation of ryanodine-sensitive calcium-release channel activity regulation of proteasomal protein catabolic process regulation of protein processing positive regulation of cell cycle arrest cellular response to glucose stimulus cellular response to parathyroid hormone stimulus cellular response to glucagon stimulus cellular response to epinephrine stimulus cell communication by electrical coupling involved in cardiac conduction negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning regulation of cardiac conduction regulation of bicellular tight junction assembly signal transduction adenylate cyclase-modulating G-protein coupled receptor signaling pathway response to clozapine spermatogenesis male gonad development modulation of synaptic transmission phosphorylation
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