Invitrogen

Phospho-PKC beta-2 (Thr641) Polyclonal Antibody

Phospho-PKC beta-2 (Thr641) Antibody in Western Blot (WB)

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Phospho-PKC beta-2 (Thr641) Antibody (44-964G) in WB

PKC-beta II (pT641) phosphospecific antibody. PMA-treated K652 cell lysates were incubated with anti-PKC-beta II (pT641) after prior incubation with: no peptide (1, 2), the non-phosphopeptide (3), a generic (pT) peptide (4), or, the phosphopeptide immunogen (5). Peptide competition data show antibody phospho-site specificity. Lane 1 was treated with l phosphatase prior to immunoblot. Absence of signal furth... View More

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产品信息

44-964G

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化 (IHC)

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产品规格

种属反应

Human

已发表种属

Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human PKCbII that contains threonine 641. The sequence is conserved in mouse and rat.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533810

靶标信息

The PKC family of serine/threonine kinases, including PRKCB2 (PKC beta II), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Tiron ameliorates high glucose-induced cardiac myocyte apoptosis by PKCδ-dependent inhibition of osteopontin.

Clinical and experimental pharmacology & physiology

Jiang P,Zhang D,Qiu H,Yi X,Zhang Y,Cao Y,Zhao B,Xia Z,Wang C

44-964G was used in Western Blot to indicate that ROS-mediated activation of PKCδ upregulated OPN expression, leading to cardiac myocyte apoptosis. Interfering with ROS/PKCδ pathway by antioxidants such as tiron provides an optional therapeutic strategy for treatment and prevention of apoptosis-related cardiovascular diseases including diabetic cardiomyopathy.