Invitrogen

Phospho-PKC lambda/iota (Thr557, Thr564) Polyclonal Antibody

Phospho-PKC lambda/iota (Thr557, Thr564) Antibody in Western Blot (WB)

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Phospho-PKC lambda/iota (Thr557, Thr564) Antibody (44-968G) in WB

Lysates prepared from Jurkat cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda phosphatase (5), blocked with a 5% low-fat milk-TBST buffer for one hour at room temperature, and incubated with PKCiota (pT555) antibody for two hours at room temperature in a 3% low-fat milk-TBST buffer, foll... View More

Phospho-PKC lambda/iota (Thr557, Thr564) Antibody

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产品信息

44-968G

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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产品规格

种属反应

Human, Mouse

已发表种属

Dog, Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human PKCi that contains threonine 557 and 564 (phosphorylated). The sequence is conserved in mouse PKCi and human PKCl.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533812

靶标信息

The PKC family of serine/threonine kinases, including PRKCI (PKC iota), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Oncogenic PKC-ι activates Vimentin during epithelial-mesenchymal transition in melanoma; a study based on PKC-ι and PKC-ζ specific inhibitors.

Cell adhesion & migration

Ratnayake WS,Apostolatos CA,Apostolatos AH,Schutte RJ,Huynh MA,Ostrov DA,Acevedo-Duncan M

Published figure using Phospho-PKC lambda/iota (Thr555, Thr563) polyclonal antibody (Product # 44-968G) in Western Blot

Mon Mar 09 00:00:00 UTC 2020

Mature induced-pluripotent-stem-cell-derived human podocytes reconstitute kidney glomerular-capillary-wall function on a chip.

Nature biomedical engineering

Musah S,Mammoto A,Ferrante TC,Jeanty SSF,Hirano-Kobayashi M,Mammoto T,Roberts K,Chung S,Novak R,Ingram M,Fatanat-Didar T,Koshy S,Weaver JC,Church GM,Ingber DE

44-968G was used in Western Blotting to create a glomerulus-on-a-chip in vitro model which can mimic adriamycin-induced albuminuria and podocyte injury.

Wed Nov 20 00:00:00 UTC 2019

Two novel atypical PKC inhibitors; ACPD and DNDA effectively mitigate cell proliferation and epithelial to mesenchymal transition of metastatic melanoma while inducing apoptosis.

International journal of oncology

Ratnayake WS,Apostolatos AH,Ostrov DA,Acevedo-Duncan M

Wed Nov 01 00:00:00 UTC 2017

Regulation of polarized morphogenesis by protein kinase C iota in oncogenic epithelial spheroids.

Carcinogenesis

Linch M,Sanz-Garcia M,Rosse C,Riou P,Peel N,Madsen CD,Sahai E,Downward J,Khwaja A,Dillon C,Roffey J,Cameron AJ,Parker PJ

44968G was used in western blot to determine the contribution of protein kinase C iota to normal-polarized mammalian morphogenesis

Sat Feb 01 00:00:00 UTC 2014

生物信息学

蛋白别名: aPKC-lambda/iota; Atypical protein kinase C-lambda/iota; nPKC-iota; PRKC-lambda/iota; Protein kinase C iota type; protein kinase C, iota; protein kinase C, lambda

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基因别名: 2310021H13Rik; AI427505; aPKClambda; DXS1179E; mKIAA4165; nPKC-iota; PKCI; Pkcl; PKClambda; PRKCI; Prkcl

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UniProt ID: (Human) P41743, (Mouse) Q62074

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Entrez Gene ID: (Human) 5584, (Mouse) 18759

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功能