Invitrogen

Phospho-PLCG1 (Tyr783) Polyclonal Antibody

Phospho-PLCG1 (Tyr783) Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 12

Phospho-PLCG1 (Tyr783) Antibody (44-696G) in ICC/IF

PLC-1 (pY783) phosphospecific antibody, Colocalized detection of PLC-1 (pY783) (green) and actin stress fibers (red) at the sites of membrane ruffles and focal adhesions in fibroblasts.

Phospho-PLCG1 (Tyr783) Antibody in Western Blot (WB)

Product Image Gallery

产品信息

44-696G

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看17篇已发表文章 17篇已发表文章

免疫组化 (IHC)

Assay-dependent

免疫细胞化学 (ICC/IF)

Assay-dependent

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

查看2篇已发表文章 2篇已发表文章

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human PLC-gamma-1 that contains tyrosine 783. This region is conserved among many species including mouse, rat, cow and frog.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533725

靶标信息

The mammalian Phospholipase Cg (PLCg) family has two closely related proteins, PLCg1 and PLCg2. The PLCg isozymes have the core structure domains and a unique array of domains containing an additional PH domain, two SH2 domains and one SH3 domain. In response to extracellular stimuli, such as hormones and growth factors, receptor tyrosine kinases (RTKs) phosphorylate and activate PLCg. Activated PLCg catalyzes hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) to produce the metabolic second messenger molecules inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Paladin is a phosphoinositide phosphatase regulating endosomal VEGFR2 signalling and angiogenesis.

EMBO reports

Nitzsche A,Pietilä R,Love DT,Testini C,Ninchoji T,Smith RO,Ekvärn E,Larsson J,Roche FP,Egaña I,Jauhiainen S,Berger P,Claesson-Welsh L,Hellström M

Published figure using Phospho-PLCG1 (Tyr783) polyclonal antibody (Product # 44-696G) in Western Blot

Wed Feb 03 00:00:00 UTC 2021

Downregulation of PUMA underlies resistance to FGFR1 inhibitors in the stem cell leukemia/lymphoma syndrome.

Cell death & disease

Liu Y,Cai B,Chong Y,Zhang H,Kemp CA,Lu S,Chang CS,Ren M,Cowell JK,Hu T

44-696G was used in Western Blotting to show resistance to FGFR1 inhibitors in stem cell leukemia/lymphoma syndrome is promoted by downregulation of the protein p53 upregulated modulator of apoptosis.

Tue Oct 20 00:00:00 UTC 2020

Downregulation of PUMA underlies resistance to FGFR1 inhibitors in the stem cell leukemia/lymphoma syndrome.

Cell death & disease

Liu Y,Cai B,Chong Y,Zhang H,Kemp CA,Lu S,Chang CS,Ren M,Cowell JK,Hu T

Tue Oct 20 00:00:00 UTC 2020

ADAP is an upstream regulator that precedes SLP-76 at sites of TCR engagement and stabilizes signaling microclusters.

Journal of cell science

Lewis JB,Scangarello FA,Murphy JM,Eidell KP,Sodipo MO,Ophir MJ,Sargeant R,Seminario MC,Bunnell SC

44-696G was used in Western Blotting to show that the interaction of the adhesion and degranulation-promoting adaptor protein with SLP-76 enables the formation of persistent microclusters and the stabilization of T cell contacts and enables the upregulation of CD69.

Thu Nov 08 00:00:00 UTC 2018

THEMIS enhances TCR signaling and enables positive selection by selective inhibition of the phosphatase SHP-1.

Nature immunology

Choi S,Warzecha C,Zvezdova E,Lee J,Argenty J,Lesourne R,Aravind L,Love PE

44-696G was used in Western Blotting to identify that THEMIS facilitates thymocyte positive selection by enhancing the T cell antigen receptor signaling response to low-affinity ligands.

Sat Apr 01 00:00:00 UTC 2017

BDNF-TrkB controls cocaine-induced dendritic spines in rodent nucleus accumbens dissociated from increases in addictive behaviors.

Proceedings of the National Academy of Sciences of the United States of America

Anderson EM,Wissman AM,Chemplanikal J,Buzin N,Guzman D,Larson EB,Neve RL,Nestler EJ,Cowan CW,Self DW

44-696G was used in Western Blotting to indicate that BDNF-TrkB signaling both mediates and reverses cocaine-induced increases in dendritic spine density in NACsh neurons, and these morphological changes are entirely dissociable from changes in addictive behavior.

Tue Aug 29 00:00:00 UTC 2017

RasGRP1 overexpression in T-ALL increases basal nucleotide exchange on Ras rendering the Ras/PI3K/Akt pathway responsive to protumorigenic cytokines.

Oncogene

Ksionda O,Melton AA,Bache J,Tenhagen M,Bakker J,Harvey R,Winter SS,Rubio I,Roose JP

44-696G was used in western blot to elucidate the contribution of leukemogenic Ras signals

Thu Jul 14 00:00:00 UTC 2016

LynA regulates an inflammation-sensitive signaling checkpoint in macrophages.

eLife

Freedman TS,Tan YX,Skrzypczynska KM,Manz BN,Sjaastad FV,Goodridge HS,Lowell CA,Weiss A

44-696G was used in western blot to determine the response threshold of immunoreceptor tyrosine-based activation motifs

Fri Oct 30 00:00:00 UTC 2015

Small molecule inhibition of Csk alters affinity recognition by T cells.

eLife

Manz BN,Tan YX,Courtney AH,Rutaganira F,Palmer E,Shokat KM,Weiss A

Published figure using Phospho-PLCG1 (Tyr783) polyclonal antibody (Product # 44-696G) in Western Blot

Mon Aug 24 00:00:00 UTC 2015

Inhibition of the kinase Csk in thymocytes reveals a requirement for actin remodeling in the initiation of full TCR signaling.

Nature immunology

Tan YX,Manz BN,Freedman TS,Zhang C,Shokat KM,Weiss A

Published figure using Phospho-PLCG1 (Tyr783) polyclonal antibody (Product # 44-696G) in Western Blot

Sat Feb 01 00:00:00 UTC 2014

Pak2 is required for actin cytoskeleton remodeling, TCR signaling, and normal thymocyte development and maturation.

eLife

Phee H,Au-Yeung BB,Pryshchep O,O'Hagan KL,Fairbairn SG,Radu M,Kosoff R,Mollenauer M,Cheng D,Chernoff J,Weiss A

44-696G was used in western blot to study the role of P21-activated kinase 2 in the immune system

Tue May 13 00:00:00 UTC 2014

Tetraspanin CD63 promotes vascular endothelial growth factor receptor 2-β1 integrin complex formation, thereby regulating activation and downstream signaling in endothelial cells in vitro and in vivo.

The Journal of biological chemistry

Tugues S,Honjo S,König C,Padhan N,Kroon J,Gualandi L,Li X,Barkefors I,Thijssen VL,Griffioen AW,Claesson-Welsh L

Fri Jun 28 00:00:00 UTC 2013

Quantitative differences in CD45 expression unmask functions for CD45 in B-cell development, tolerance, and survival.

Proceedings of the National Academy of Sciences of the United States of America

Zikherman J,Doan K,Parameswaran R,Raschke W,Weiss A

Tue Jan 03 00:00:00 UTC 2012

Quantitative differences in CD45 expression unmask functions for CD45 in B-cell development, tolerance, and survival.

Proceedings of the National Academy of Sciences of the United States of America

Zikherman J,Doan K,Parameswaran R,Raschke W,Weiss A

Tue Jan 03 00:00:00 UTC 2012

PEST motif serine and tyrosine phosphorylation controls vascular endothelial growth factor receptor 2 stability and downregulation.

Molecular and cellular biology

Meyer RD,Srinivasan S,Singh AJ,Mahoney JE,Gharahassanlou KR,Rahimi N

Sun May 01 00:00:00 UTC 2011

PEST motif serine and tyrosine phosphorylation controls vascular endothelial growth factor receptor 2 stability and downregulation.

Molecular and cellular biology

Meyer RD,Srinivasan S,Singh AJ,Mahoney JE,Gharahassanlou KR,Rahimi N

Sun May 01 00:00:00 UTC 2011

PEST motif serine and tyrosine phosphorylation controls vascular endothelial growth factor receptor 2 stability and downregulation.

Molecular and cellular biology

Meyer RD,Srinivasan S,Singh AJ,Mahoney JE,Gharahassanlou KR,Rahimi N

Sun May 01 00:00:00 UTC 2011

Vitamin D controls T cell antigen receptor signaling and activation of human T cells.

Nature immunology

von Essen MR,Kongsbak M,Schjerling P,Olgaard K,Odum N,Geisler C

44-696G was used in western blot to explore the relationship between PLC-gamma1 and TCR

Thu Apr 01 00:00:00 UTC 2010

Stability of an autoinhibitory interface in the structure of the tyrosine kinase ZAP-70 impacts T cell receptor response.

Proceedings of the National Academy of Sciences of the United States of America

Deindl S,Kadlecek TA,Cao X,Kuriyan J,Weiss A

Tue Dec 08 00:00:00 UTC 2009

Paxillin-Y118 phosphorylation contributes to the control of Src-induced anchorage-independent growth by FAK and adhesion.

BMC cancer

Sachdev S,Bu Y,Gelman IH

44-696G was used in western blot to review the functional consequences of interactions between focal adhesion kinase and Src are protein tyrosine kinases

Mon Jan 12 00:00:00 UTC 2009

Intramolecular regulatory switch in ZAP-70: analogy with receptor tyrosine kinases.

Molecular and cellular biology

Brdicka T,Kadlecek TA,Roose JP,Pastuszak AW,Weiss A

Wed Jun 01 00:00:00 UTC 2005

Functional tyrosine kinase inhibitor profiling: a generally applicable method points to a novel role of platelet-derived growth factor receptor-beta in tuberous sclerosis.

The American journal of pathology

Arbiser JL,Govindarajan B,Bai X,Onda H,Kazlauskas A,Lim SD,Amin MB,Claesson-Welsh L

Sun Sep 01 00:00:00 UTC 2002

生物信息学

蛋白别名: 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1; cell differentiation and embryonic development; EC 3.1.4.11; inositoltrisphosphohydrolase; monophosphatidylinositol phosphodiesterase; OTTHUMP00000178982; phosphatidylinositol phospholipase C; phosphoinositidase C; phosphoinositide phospholipase C; Phosphoinositide phospholipase C-gamma-1; phospholipase C, gamma 1 (formerly subtype 148); phospholipase C-1; phospholipase C-148; Phospholipase C-gamma-1; Phospholipase C-II; Phospholipase C2; PLC gamma1; PLC-148; PLC-gamma-1; PLC-II; PLC2I; triphosphoinositide phosphodiesterase

查看更多收起

基因别名: AI894140; Cded; NCKAP3; Plc-1; Plc-gamma1; PLC-II; PLC1; PLC148; Plcg-1; PLCG1; PLCgamma1

查看更多收起

UniProt ID: (Human) P19174, (Mouse) Q62077

查看更多收起

Entrez Gene ID: (Human) 5335, (Mouse) 18803

查看更多收起

功能