Invitrogen

Phospho-PLCG2 (Tyr759) Monoclonal Antibody (4NPRN4), PE, eBioscience™

Phospho-PLCG2 (Tyr759) Antibody in Flow Cytometry (Flow)

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Phospho-PLCG2 (Tyr759) Antibody (12-9866-42) in Flow

Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 minutes with F(ab')2-Goat anti-Human IgG, IgM (H+L) Secondary Antibody, Functional Grade (Product # 16-5099-85) (right). Cells were then stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with CD19 Monoclonal Antibody, APC (Product # 17-0199-82) and Phosp... View More

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产品信息

12-9866-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.06 µg)/test

产品规格

种属反应

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

4NPRN4

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2723469

产品详细信息

Applications Reported: This 4NPRN4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 4NPRN4 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser

靶标信息

Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes, including PLC beta1, PLC beta2, PLC beta3, PLC beta4, PLC gamma1, PLC gamma2, PLC delta1, PLC delta2 and PLCe. PLC delta exists as four different isoforms. PLC delta1, a calcium signal amplifier, is activated by an atypical GTP-binding protein. In addition, PLC delta1 is an effector for GTP-binding protein transglutaminase II-mediated oxytocin receptor and alpha1B-adrenoreceptor signaling. Mouse PLC delta1 is highly expressed in brain, heart, lung and testis. PLC delta is abnormally accumulated in autopsied brains with Alzheimer's disease (AD), suggesting that it may play a role in the pathology of AD. PLC delta2 is markedly expressed in type II intestinal metaplasia and in the adenocarcinoma. When PLC delta2 is expressed in type I intestinal metaplasia, the metaplasia is generally considered benignant, yet evolves toward neoplastic transformation. Thus, PLC delta2 expression may be a possible marker of gastric malignant transformation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2; Phosphoinositide phospholipase C-gamma-2; phospholipase C, gamma 2 (phosphatidylinositol-specific); Phospholipase C-gamma-2; Phospholipase C-IV; PLC gamma 2; PLC gamma-2; PLC gamma2; PLC- gamma2; PLC-gamma; PLC-gamma-2; PLC-IV

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基因别名: APLAID; FCAS3; PLC-gamma-2; PLC-IV; Plcg-2; PLCG2; PLCgamma2

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UniProt ID: (Human) P16885, (Mouse) Q8CIH5

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Entrez Gene ID: (Human) 5336, (Mouse) 234779

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功能

phosphatidylinositol phospholipase C activity phospholipase C activity signal transducer activity protein binding phosphoric diester hydrolase activity hydrolase activity phospholipase

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