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Invitrogen
Phospho-PLCG2 (Tyr759) Monoclonal Antibody (4NPRN4), PerCP-eFluor™ 710, eBioscience™
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Phospho-PLCG2 (Tyr759) Antibody (46-9866-42) in Flow
Normal human peripheral blood cells cells were unstimulated (left) or stimulated for 5 minutes with F(ab')2-Goat anti-Human IgG, IgM (H+L) Secondary Antibody, Functional Grade (Product # 16-5099-85) (right). Cells were then stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with CD19 Monoclonal Antibody, APC (Product # 17-0199-82) and... View More
产品信息
46-9866-42
产品规格
种属反应
Human,
Mouse
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
4NPRN4
偶联物
激发/发射光谱
482/708 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2723472
产品详细信息
Applications Reported: This 4NPRN4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 4NPRN4 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
靶标信息
Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes, including PLC beta1, PLC beta2, PLC beta3, PLC beta4, PLC gamma1, PLC gamma2, PLC delta1, PLC delta2 and PLCe. PLC delta exists as four different isoforms. PLC delta1, a calcium signal amplifier, is activated by an atypical GTP-binding protein. In addition, PLC delta1 is an effector for GTP-binding protein transglutaminase II-mediated oxytocin receptor and alpha1B-adrenoreceptor signaling. Mouse PLC delta1 is highly expressed in brain, heart, lung and testis. PLC delta is abnormally accumulated in autopsied brains with Alzheimer's disease (AD), suggesting that it may play a role in the pathology of AD. PLC delta2 is markedly expressed in type II intestinal metaplasia and in the adenocarcinoma. When PLC delta2 is expressed in type I intestinal metaplasia, the metaplasia is generally considered benignant, yet evolves toward neoplastic transformation. Thus, PLC delta2 expression may be a possible marker of gastric malignant transformation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2; Phosphoinositide phospholipase C-gamma-2; phospholipase C, gamma 2 (phosphatidylinositol-specific); Phospholipase C-gamma-2; Phospholipase C-IV; PLC gamma 2; PLC gamma-2; PLC gamma2; PLC- gamma2; PLC-gamma; PLC-gamma-2; PLC-IV
基因别名: APLAID; FCAS3; PLC-gamma-2; PLC-IV; Plcg-2; PLCG2; PLCgamma2
UniProt ID: (Human) P16885, (Mouse) Q8CIH5
Entrez Gene ID: (Human) 5336, (Mouse) 234779
positive regulation of receptor internalization
stimulatory C-type lectin receptor signaling pathway
follicular B cell differentiation
phosphatidylinositol biosynthetic process
phospholipid catabolic process
regulation of gene expression
Wnt signaling pathway
calcium-mediated signaling
platelet activation
B cell differentiation
activation of store-operated calcium channel activity
positive regulation of type I interferon production
response to lipopolysaccharide
inositol trisphosphate biosynthetic process
Fc-epsilon receptor signaling pathway
Fc-gamma receptor signaling pathway involved in phagocytosis
negative regulation of programmed cell death
inositol phosphate metabolic process
T cell receptor signaling pathway
B cell receptor signaling pathway
release of sequestered calcium ion into cytosol
lipid metabolic process
signal transduction
positive regulation of cytosolic calcium ion concentration
lipid catabolic process
response to magnesium ion
response to ATP
intracellular signal transduction
