Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 3
Phospho-PRAK (Ser354) Antibody (PA5-105676) in ICC/IF
Immunocytochemistry analysis of Phospho-PRAK (Ser354) in HepG2 cells (serum starvation). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-PRAK (Ser354) polyclonal antibody (Product # PA5-105676) 1:200 and mouse anti-beta tubulin antibody (1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat an... View More
产品信息
PA5-105676
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human MAPKAPK5(Accession Q8IW41), corresponding to amino acid residues around phosphorylated Ser354.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
sequential chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2817104
产品详细信息
Antibody detects endogenous levels of MAPKAPK5 only when phosphorylated at Ser354.
靶标信息
MAPKAPK5 (PRAK) is activated in response to cellular stress and pro-inflammatory cytokines. Activity of this kinase is regulated by p38 alpha and p38 beta. The protein encoded by PRAK is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of PRAK encoding distinct isoforms have been reported.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: MAP kinase-activated protein kinase 5; MAPK-activated protein kinase 5; MAPKAP kinase 5; MAPKAPK-5; p38-regulated/activated protein kinase; PRAK
基因别名: MAPKAP-K5; MAPKAPK5; MK-5; MK5; PRAK
UniProt ID: (Human) Q8IW41, (Mouse) O54992
Entrez Gene ID: (Human) 8550, (Rat) 498183, (Mouse) 17165
p53 binding
protein serine/threonine kinase activity
calmodulin-dependent protein kinase activity
MAP kinase kinase activity
protein binding
calmodulin binding
ATP binding
calcium-dependent protein serine/threonine kinase activity
protein kinase activity
mitogen-activated protein kinase binding
nucleotide binding
signal transducer activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
MAPK cascade
activation of MAPK activity
regulation of translation
signal transduction
Ras protein signal transduction
peptidyl-serine phosphorylation
negative regulation of TOR signaling
positive regulation of telomere maintenance via telomerase
protein autophosphorylation
positive regulation of telomerase activity
stress-induced premature senescence
regulation of signal transduction by p53 class mediator
positive regulation of telomere capping
protein phosphorylation
cell surface receptor signaling pathway
phosphorylation
