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Invitrogen
Phospho-PRKAR2A (Ser99) Recombinant Rabbit Monoclonal Antibody (SC54-04)
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Phospho-PRKAR2A (Ser99) Antibody (MA5-32203) in ICC/IF
Immunocytochemical analysis of Phospho-PRKAR2A (Ser99) in MCF-7 cells using a Phospho-PRKAR2A (Ser99) Monoclonal antibody (Product # MA5-32203) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
产品信息
MA5-32203
产品规格
种属反应
Human,
Mouse,
Pig,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
SC54-04
抗原
Synthetic phospho-peptide corresponding to residues surrounding Ser99 of Human PKA R2 aa 82-126
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2809490
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of AMPK.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: cAMP-dependent protein kinase regulatory subunit RII alpha; cAMP-dependent protein kinase type II-alpha regulatory subunit; cAMP-dependent protein kinase, regulatory subunit alpha 2; MGC3606; protein kinase A, RII-alpha subunit; protein kinase cAMP-dependent regulatory type II alpha; protein kinase, cAMP-dependent, regulatory subunit type II alpha; protein kinase, cAMP-dependent, regulatory, type 2, alpha; unnamed protein product
基因别名: 1110061A24Rik; AI317181; AI836829; PKR2; PRKAR2; PRKAR2A; RII(alpha)
UniProt ID: (Human) P13861, (Rat) P12368
Entrez Gene ID: (Pig) 397493, (Human) 5576, (Mouse) 19087, (Rat) 29699
protein binding
cAMP binding
cAMP-dependent protein kinase inhibitor activity
cAMP-dependent protein kinase regulator activity
ubiquitin protein ligase binding
protein kinase A catalytic subunit binding
nucleotide binding
protein domain specific binding
small molecule binding
protein homodimerization activity
kinase activity
protein-binding activity modulator
kinase modulator
regulation of meiotic cell cycle process involved in oocyte maturation
positive regulation of meiotic cell cycle process involved in oocyte maturation
negative regulation of cAMP-dependent protein kinase activity
renal water homeostasis
blood coagulation
activation of protein kinase A activity
intracellular signal transduction
cellular response to glucagon stimulus
regulation of protein phosphorylation
regulation of protein kinase activity
protein heterooligomerization
phosphorylation
response to clozapine
