Invitrogen

Phospho-Paxillin (Ser83) Polyclonal Antibody

Phospho-Paxillin (Ser83) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-Paxillin (Ser83) Antibody (PA5-143736) in ICC/IF

Immunocytochemistry analysis of Phospho-Paxillin (Ser83) in rabbit spleen fibroblasts. The cells were labeled with mouse Paxillin monoclonal antibody (left) and rabbit Phospho-Paxillin (Ser83) polyclonal antibody (Product # PA5-143736) (right), then detected using appropriate secondary antibodies conjugated to Cy3.

Phospho-Paxillin (Ser83) Antibody in Western Blot (WB)

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产品信息

PA5-143736

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫细胞化学 (ICC/IF)

1:500

产品规格

种属反应

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Phospho-Paxillin (Ser-83) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 83 of mouse paxillin. This sequence is highly conserved in rat and human paxillin and is also found in all isoforms (a, b, g) of paxillin.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA, 50% glycerol

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2942964

产品详细信息

The antibody detects a 68kDa* protein corresponding to the molecular mass of phosphorylated paxillin on SDS-PAGE immunoblots of NGF treated rat PC12 cells, as well as calyculin A treated human A431 cells and mouse macrophages.

靶标信息

Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。