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Phospho-RRAS (Tyr66) Antibody (PA5-118783) in IHC (P)
Immunohistochemical analysis of Phospho-RRAS (Tyr66) in Human gastric cancer and adjacent normal tissues using a Phospho-RRAS (Tyr66) polyclonal antibody (Product # PA5-118783). The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibo... View More
产品信息
PA5-118783
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human RRAS(Accession P10301), corresponding to amino acid residues around phosphorylated Tyr66.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
sequential chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2903283
产品详细信息
Antibody detects endogenous levels of R-Ras only when phosphorylated at Tyr66.
靶标信息
The Ras superfamily of small GTPases includes the Ras, Rho, Arf, Rab, and Ran families. Ras-regulated signal pathways control such as actin cytoskeletal integrity, proliferation, differentiation, cell adhesion, apoptosis, and cell migration. These GTPases function as molecular switches that control eukaryotic cell function by cycling between two interconvertible forms, a GDP-bound inactive form and a GTP-bound active form. The binding of GTP leads to a conformational change in the downstream effector-binding domain of the G-protein. The small GTPases are monomeric G-proteins with molecular masses over the range 20-30 kDa. Ras is attached to the cell membrane by prenylation. Mutations in the Ras family of proto-oncogenes (comprising H-Ras, N-Ras and K-Ras) are very common, being found in 20% to 30% of all human tumours. The frequency of RAS mutations is among the highest for any gene in human cancer. K-ras mutations occur frequently in non-small-cell lung, colorectal, and pancreatic carcinomas; H-ras mutations are common in bladder, kidney, and thyroid carcinomas; N-ras mutations are found in melanoma, hepatocellular carcinoma, and hematologic malignancies.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Harvey rat sarcoma oncogene, subgroup R; Harvey rat sarcoma virus oncogene, subgroup R; oncogene RRAS; p23; ras family small GTP binding protein R-Ras; Ras-related protein R-Ras; Ras-related protein R-RAS (P23)
基因别名: AI573426; p23; RRAS
UniProt ID: (Human) P10301, (Rat) D3Z8L7, (Mouse) P10833
Entrez Gene ID: (Human) 6237, (Rat) 361568, (Mouse) 20130
leukocyte differentiation
Ras protein signal transduction
negative regulation of cell migration
positive regulation of angiogenesis
regulation of protein kinase B signaling
face morphogenesis
regulation of ERK1 and ERK2 cascade
small GTPase mediated signal transduction
metabolic process
signal transduction
