Invitrogen

Phospho-Rb (Ser249, Thr252) Polyclonal Antibody

Phospho-Rb (Ser249, Thr252) Antibody in Western Blot (WB)

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Phospho-Rb (Ser249, Thr252) Antibody (44-584G) in WB

Peptide Competition. Extracts of Jurkat cells in high growth phase were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% milk-TBST buffer for one hour at room temperature, then incubated with the Rb (pSpT249/252) antibody for two hours at room temperature in a 1% milk-TBST buffer following its prior incubation with: no peptide (1), a generic phosphos... View More

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产品信息

44-584G

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化（石蜡） (IHC (P))

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酶联免疫吸附实验 (ELISA)

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human Rb that contains serine 249 and threonine 252 (based on Swiss Protein database, accession number P06400). The sequence is conserved in human, mouse and rat.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533686

靶标信息

Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Hypophosphorylated pRb knock-in mice exhibit hallmarks of aging and vitamin C-preventable diabetes.

The EMBO journal

Jiang Z,Li H,Schroer SA,Voisin V,Ju Y,Pacal M,Erdmann N,Shi W,Chung PED,Deng T,Chen NJ,Ciavarra G,Datti A,Mak TW,Harrington L,Dick FA,Bader GD,Bremner R,Woo M,Zacksenhaus E

44-584G was used in Immunohistochemistry (Paraffin) to show that homozygous RbδK4 and RbδK7 knock-in mice, in which either four or all seven phosphorylation sites in the C-terminal region of pRb, respectively, have been abolished by Ser/Thr-to-Ala substitutions, undergo normal embryogenesis and early development, notwithstanding suppressed phosphorylation of additional upstream sites.

Tue Feb 15 00:00:00 UTC 2022

Serine 13 of the human cytomegalovirus viral cyclin-dependent kinase UL97 is required for regulatory protein 14-3-3 binding and UL97 stability.

The Journal of biological chemistry

Iwahori S,Umaña AC,Kalejta RF,Murata T

44-584G was used in Western Blotting to conclude that 14-3-3 proteins interact with and stabilize UL97 during HCMV infection.

Tue Nov 01 00:00:00 UTC 2022

Dual targeting of CDK and tropomyosin receptor kinase families by the oral inhibitor PHA-848125, an agent with broad-spectrum antitumor efficacy.

Molecular cancer therapeutics

Albanese C,Alzani R,Amboldi N,Avanzi N,Ballinari D,Brasca MG,Festuccia C,Fiorentini F,Locatelli G,Pastori W,Patton V,Roletto F,Colotta F,Galvani A,Isacchi A,Moll J,Pesenti E,Mercurio C,Ciomei M

Sun Aug 01 00:00:00 UTC 2010

Transient treatment with CDK inhibitors eliminates proliferative potential even when their abilities to evoke apoptosis and DNA damage are blocked.

Cell cycle (Georgetown, Tex.)

Scrace SF,Kierstan P,Borgognoni J,Wang LZ,Denny S,Wayne J,Bentley C,Cansfield AD,Jackson PS,Lockie AM,Curtin NJ,Newell DR,Williamson DS,Moore JD

44-584G was used in ELISA to find that many literature CDK inhibitors do not effectively suppress the CDK/cyclin complexes responsible for cell cycle progression at the minimum doses required to block proliferation: some are only effective after a substantial delay and may act via inhibition of CDK7.

Mon Dec 15 00:00:00 UTC 2008

Maid (GCIP) is involved in cell cycle control of hepatocytes.

Hepatology (Baltimore, Md.)

Sonnenberg-Riethmacher E,Wüstefeld T,Miehe M,Trautwein C,Riethmacher D

Thu Feb 01 00:00:00 UTC 2007

生物信息学

蛋白别名: Cleaved Rb; delta RB-p70; DRb-p70; exon 17 tumor GOS561 substitution mutation causes premature stop; GOS563 exon 17 substitution mutation causes premature stop; p105-Rb; P105RB; p110-RB1; Phospho-Retinoblastoma; pp110; pRb; prepro-retinoblastoma-associated protein; protein phosphatase 1, regulatory subunit 130; Rb; Rb-p70; Retin; Retinoblastoma; retinoblastoma 1; retinoblastoma gene; retinoblastoma suspectibility protein; Retinoblastoma-associated protein

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基因别名: OSRC; p105-Rb; pp110; PPP1R130; pRb; RB; RB1

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UniProt ID: (Human) P06400

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Entrez Gene ID: (Human) 5925

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功能