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Phospho-SKP2 (Thr417) Antibody (PA5-105634) in ICC/IF
Immunocytochemistry analysis of Phospho-SKP2 (Thr417) in HepG2 cells (serum starvation). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-SKP2 (Thr417) polyclonal antibody (Product # PA5-105634) 1:200 and mouse anti-beta tubulin antibody (1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat an... View More
产品信息
PA5-105634
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human SKP2(Accession Q13309), corresponding to amino acid residues around phosphorylated Thr417.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
sequential chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2817062
产品详细信息
Antibody detects endogenous levels of SKP2/p45 only when phosphorylated at Thr417.
靶标信息
Skp2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the Cyclin A-Cdk2 complex. Skp2 is essential for the G1-S transition in both transformed cells and diploid fibroblasts. Biochemical and genetic experiments have demonstrated that Skp2 is required for the ubiquitination and consequent degradation of p27 in cultured mammalian cells and in vitro reconstitution assays. In normal tissues, Skp2 is expressed in tonsil and placenta. In tumor tissues, Skp2 is expressed widely, in some colon, prostate, pancreas, and skin cancers, with high expression in lung, breast, ovarian, and endometrial cancers. Research suggests that Skp2 plays a role in oncogenesis, particularly in the pathogenesis of lymphomas and breast cancers.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CDK2/cyclin A-associated protein p45; cyclin A/CDK2-associated protein p45; Cyclin-A/CDK2-associated protein p45; F-box protein Skp2; F-box/LRR-repeat protein 1; F-box/WD-40 protein 1; FWD1; MGC1366; OTTHUMP00000221389; p45skp2; S-phase kinase-associated protein 2; S-phase kinase-associated protein 2 (p45)
基因别名: 4930500A04Rik; FBL1; FBXL1; FLB1; FWD1; p45; RGD1562456; SKP2
UniProt ID: (Human) Q13309, (Mouse) Q9Z0Z3
Entrez Gene ID: (Human) 6502, (Rat) 294790, (Mouse) 27401
G1/S transition of mitotic cell cycle
G2/M transition of mitotic cell cycle
protein polyubiquitination
ubiquitin-dependent protein catabolic process
phosphorylation
positive regulation of intracellular estrogen receptor signaling pathway
regulation of apoptotic process
positive regulation of smooth muscle cell proliferation
regulation of cell cycle
cellular response to cell-matrix adhesion
positive regulation of protein polyubiquitination
cell proliferation
anaphase-promoting complex-dependent catabolic process
