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Phospho-SOX2 (Thr118) Antibody (PA5-143769) in ICC/IF
Immunocytochemistry analysis of Phospho-SOX2 (Thr118) in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with rabbit Phospho-SOX2 (Thr118) polyclonal antibody (Product # PA5-143769). The antibody was detected using goat anti-rabbit DyLight® 594.
产品信息
PA5-143769
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Phospho-Sox2 (Thr-118) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Thr-118 in mouse Sox2. This site is well conserved in rat and human Sox2, and has high homology with the conserved site in Sox1 (Thr-126) and Sox3 (Thr-144), but low homology to other Sox family members.
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA, 50% glycerol
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2942997
产品详细信息
The antibody was cross adsorbed to unphosphorylated Sox2 (Thr-118) peptide before affinity purification using phospho-Sox2 (Thr-118) peptide. This antibody antibody detects a 34 kDa* protein on SDS-PAGE immunoblots of mouse F9 stem cells. This reactivity is not observed after lambda phosphatase dephosphorylation of Sox2.
靶标信息
SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Delta EF2a; MCOPS3 (Microphthalmia Syndromic type 3); sex determining region Y-box 2; SOX 2; SRY (sex determining region Y) box 2; SRY (sex determining region Y)-box 2; SRY box 2; SRY related HMG box 2; SRY-box containing gene 2; SRY-related HMG-box gene 2; Transcription factor SOX-2; transcription factor SOX2
基因别名: ANOP3; lcc; MCOPS3; RGD1565646; Sox-2; SOX2; ysb
UniProt ID: (Human) P48431, (Mouse) P48432
Entrez Gene ID: (Human) 6657, (Rat) 499593, (Mouse) 20674
transcription regulatory region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
miRNA binding
sequence-specific DNA binding
transcription regulatory region DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
molecular_function
chromatin binding
transcription factor binding
protein heterodimerization activity
chromatin DNA binding
HMG box transcription factor
negative regulation of transcription from RNA polymerase II promoter
osteoblast differentiation
eye development
endodermal cell fate specification
chromatin organization
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
cell cycle arrest
response to wounding
regulation of gene expression
glial cell fate commitment
pituitary gland development
adenohypophysis development
positive regulation of cell-cell adhesion
forebrain development
somatic stem cell population maintenance
tissue regeneration
regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of MAPK cascade
positive regulation of cell differentiation
negative regulation of neuron differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
inner ear development
negative regulation of epithelial cell proliferation
response to growth factor
negative regulation of canonical Wnt signaling pathway
neuronal stem cell population maintenance
cell fate specification
positive regulation of neuroblast proliferation
regulation of transcription from RNA polymerase II promoter
sensory perception of sound
response to organic substance
stem cell population maintenance
forebrain neuron differentiation
cerebral cortex development
negative regulation of Wnt signaling pathway
male genitalia development
positive regulation of epithelial cell differentiation
olfactory placode formation
response to retinoic acid
inner ear morphogenesis
tongue development
cell fate commitment
negative regulation of cell differentiation
positive regulation of neuron differentiation
negative regulation of osteoblast differentiation
positive regulation of Notch signaling pathway
pigment biosynthetic process
lung alveolus development
embryonic organ development
anatomical structure formation involved in morphogenesis
neuron fate commitment
diencephalon morphogenesis
stem cell differentiation
regulation of neurogenesis
detection of mechanical stimulus involved in sensory perception of sound
detection of mechanical stimulus involved in equilibrioception
retina morphogenesis in camera-type eye
lens induction in camera-type eye
epithelial tube branching involved in lung morphogenesis
transcription, DNA-templated
multicellular organism development
