Invitrogen

Phospho-SP7 (Thr319) Polyclonal Antibody

Phospho-SP7 (Thr319) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-SP7 (Thr319) Antibody (PA5-105355) in ICC/IF

Immunocytochemistry analysis of Phospho-SP7 (Thr319) in H2O2 treated HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-SP7 (Thr319) polyclonal antibody (Product # PA5-105355) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Re... View More

Phospho-SP7 (Thr319) Antibody in Western Blot (WB)

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产品信息

PA5-105355

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human SP7(Accession Q8TDD2), corresponding to amino acid residues around phosphorylated Thr319.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

sequential chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2816783

产品详细信息

Antibody detects endogenous levels of osterix only when phosphorylated at Thr319.

靶标信息

SP7 is a C2H2-type zinc finger transcription factor of the SP gene family and a putative master regulator of bone cell differentiation (Gao et al., 2004 [PubMed 15474293]).

仅用于科研。不用于诊断过程。未经明确授权不得转售。