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Phospho-TOP2A (Ser1337) Antibody (PA5-118788) in ICC/IF
Immunocytochemistry analysis of Phospho-TOP2A (Ser1337) in A549 cells (UV treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-TOP2A (Ser1337) polyclonal antibody (Product # PA5-118788) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (... View More
产品信息
PA5-118788
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human TOP2A(Accession P11388), corresponding to amino acid residues around phosphorylated Ser1337.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
sequential chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2903288
产品详细信息
Antibody detects endogenous levels of TOP2A only when phosphorylated at Ser1337.
靶标信息
Topo II alpha plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2 and M-phases in transformed and developmentally regulated normal cellsTopo II alpha is also implicated in drug resistance of tumor cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DNA gyrase; DNA topoisomerase (ATP-hydrolyzing); DNA topoisomerase 2-alpha; DNA Topoisomerase II alpha; DNA topoisomerase II, 170 kD; DNA topoisomerase II, alpha isozyme; EC 5.99.1.3; OTTHUMP00000207108; P11388-1; topoisomerase (DNA) 2 alpha; topoisomerase (DNA) II alpha 170kDa; topoisomerase ii alpha
基因别名: Top-2; TOP2; TOP2A; TP2A
UniProt ID: (Human) P11388, (Mouse) Q01320
Entrez Gene ID: (Human) 7153, (Mouse) 21973, (Rat) 360243
magnesium ion binding
DNA binding
chromatin binding
DNA topoisomerase type II (ATP-hydrolyzing) activity
protein kinase C binding
protein binding
ATP binding
protein C-terminus binding
DNA-dependent ATPase activity
drug binding
DNA binding, bending
enzyme binding
protein homodimerization activity
histone deacetylase binding
ubiquitin binding
poly(A) RNA binding
protein heterodimerization activity
nucleotide binding
DNA topoisomerase activity
isomerase activity
sequence-specific DNA binding
structure-specific DNA binding
metal ion binding
DNA metabolism protein
resolution of meiotic recombination intermediates
sister chromatid segregation
hematopoietic progenitor cell differentiation
DNA topological change
DNA ligation
DNA unwinding involved in DNA replication
mitotic recombination
cellular response to DNA damage stimulus
chromosome segregation
protein sumoylation
apoptotic chromosome condensation
embryonic cleavage
regulation of circadian rhythm
positive regulation of apoptotic process
mitotic DNA integrity checkpoint
positive regulation of viral genome replication
positive regulation of single stranded viral RNA replication via double stranded DNA intermediate
positive regulation of transcription from RNA polymerase II promoter
rhythmic process
DNA metabolic process
chromosome condensation
brain development
aging
cerebellar Purkinje cell differentiation
cerebellar granule cell differentiation
response to drug
response to parathyroid hormone
