Invitrogen

Phospho-TTK (Thr33, Ser37) Polyclonal Antibody

Phospho-TTK (Thr33, Ser37) Antibody in Western Blot (WB)

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Phospho-TTK (Thr33, Ser37) Antibody (44-1325G) in WB

NIH3T3 fibroblast lysates, spiked with 1 ng active GST tagged-TTK protein (Invitrogen, Product # PV3792) (2-6) or Kinase dead mutant TTK protein (1) were resolved on a 10% polyacrylamide gel and transferred to PVDF. The membrane was blocked with a 3% BSA-TBST buffer for one hour at room temperature, and then incubated with the TTK (pTpS33/37) antibody for two hours at room temperature in 3% BSA-TBST buffer,... View More

Phospho-TTK (Thr33, Ser37) Antibody in Immunoprecipitation (IP)

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产品信息

44-1325G

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫细胞化学 (ICC/IF)

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酶联免疫吸附实验 (ELISA)

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免疫沉淀 (IP)

Assay-dependent

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其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

Human, Rat, Tag

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human TTK that contains threonine 33 and serine 37.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533594

靶标信息

TTK is a serine/threonine kinase closely related to SPK1, PIM 1 and CDC2 serine/threonine kinases. TTK is a dual-specificity kinase, which can also phosphorylate tyrosine. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. TTK encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A Biosensor for the Mitotic Kinase MPS1 Reveals Spatiotemporal Activity Dynamics and Regulation.

Current biology : CB

Kuijt TEF,Lambers MLA,Weterings S,Ponsioen B,Bolhaqueiro ACF,Staijen DHM,Kops GJPL

44-1325G was used in Immunocytochemistry to develop MPS1sen, a sensitive and specific FRET-based biosensor for MPS1 activity o understand when and where MPS1 activation occurs and how MPS1 signaling is modulated during mitosis.

Mon Oct 05 00:00:00 UTC 2020

Checkpoint signaling and error correction require regulation of the MPS1 T-loop by PP2A-B56.

The Journal of cell biology

Hayward D,Bancroft J,Mangat D,Alfonso-Pérez T,Dugdale S,McCarthy J,Barr FA,Gruneberg U

44-1325G was used in Immunocytochemistry-immunoflourescence to investigate if the BUBR1-bound pool of PP2A-B56 regulates monopolar spindle 1 T-loop autophosphorylation and hence its activation status in mammalian cells.

Mon Oct 07 00:00:00 UTC 2019

Characterisation of CCT271850, a selective, oral and potent MPS1 inhibitor, used to directly measure in vivo MPS1 inhibition vs therapeutic efficacy.

British journal of cancer

Faisal A,Mak GWY,Gurden MD,Xavier CPR,Anderhub SJ,Innocenti P,Westwood IM,Naud S,Hayes A,Box G,Valenti MR,De Haven Brandon AK,O'Fee L,Schmitt J,Woodward HL,Burke R,vanMontfort RLM,Blagg J,Raynaud FI,Eccles SA,Hoelder S,Linardopoulos S

Published figure using Phospho-TTK (Thr33, Ser37) polyclonal antibody (Product # 44-1325G) in Immunoprecipitation

Tue Apr 25 00:00:00 UTC 2017

Naturally Occurring Mutations in the MPS1 Gene Predispose Cells to Kinase Inhibitor Drug Resistance.

Cancer research

Gurden MD,Westwood IM,Faisal A,Naud S,Cheung KM,McAndrew C,Wood A,Schmitt J,Boxall K,Mak G,Workman P,Burke R,Hoelder S,Blagg J,Van Montfort RL,Linardopoulos S

44-1325G was used in immunocytochemistry to identify mutations in the kinase domain of MPS1 that confer resistance against multiple inhibitors.

Sat Aug 15 00:00:00 UTC 2015

Mitotic Checkpoint Kinase Mps1 Has a Role in Normal Physiology which Impacts Clinical Utility.

PloS one

Martinez R,Blasina A,Hallin JF,Hu W,Rymer I,Fan J,Hoffman RL,Murphy S,Marx M,Yanochko G,Trajkovic D,Dinh D,Timofeevski S,Zhu Z,Sun P,Lappin PB,Murray BW

44-1325G was used in immunocytochemistry to assess Mps1 kinase inhibitors as cancer treatments.

Tue Jun 14 00:00:00 UTC 2016

Structure-based design of orally bioavailable 1H-pyrrolo[3,2-c]pyridine inhibitors of mitotic kinase monopolar spindle 1 (MPS1).

Journal of medicinal chemistry

Naud S,Westwood IM,Faisal A,Sheldrake P,Bavetsias V,Atrash B,Cheung KM,Liu M,Hayes A,Schmitt J,Wood A,Choi V,Boxall K,Mak G,Gurden M,Valenti M,de Haven Brandon A,Henley A,Baker R,McAndrew C,Matijssen B,Burke R,Hoelder S,Eccles SA,Raynaud FI,Linardopoulos S,van Montfort RL,Blagg J

Fri Dec 27 00:00:00 UTC 2013

Structure-based design of orally bioavailable 1H-pyrrolo[3,2-c]pyridine inhibitors of mitotic kinase monopolar spindle 1 (MPS1).

Journal of medicinal chemistry

Fri Dec 27 00:00:00 UTC 2013

Structure-based design of orally bioavailable 1H-pyrrolo[3,2-c]pyridine inhibitors of mitotic kinase monopolar spindle 1 (MPS1).

Journal of medicinal chemistry

Fri Dec 27 00:00:00 UTC 2013

生物信息学

蛋白别名: cancer/testis antigen 96; Dual specificity protein kinase TTK; monopolar spindle 1 kinase; monopolar spindle 1-like 1; OTTHUMP00000016778; OTTHUMP00000220743; phosphotyrosine picked threonine kinase; Phosphotyrosine picked threonine-protein kinase; PYT

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基因别名: CT96; ESK; MPH1; MPS1; MPS1L1; PYT; TTK

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UniProt ID: (Human) P33981

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Entrez Gene ID: (Human) 7272

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功能

protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein tyrosine kinase activity protein binding ATP binding non-receptor serine/threonine protein kinase

参与通路

spindle organization mitotic spindle organization mitotic spindle assembly checkpoint positive regulation of cell proliferation positive regulation of pathway-restricted SMAD protein phosphorylation peptidyl-tyrosine phosphorylation chromosome separation